Title Paenibacillus cucumis sp. nov. Isolated from Greenhouse Soil
Author Jae-Hyung Ahn1, Byoung-chan Kim2, Byung-Yong Kim1, Soo-Jin Kim1, Jaekyeong Song1, Soon-Wo Kwon1, and Hang-Yeon Weon1*
Address 1Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea, 2Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea
Bibliography Journal of Microbiology, 52(6),460-464, 2014,
DOI 10.1007/s12275-014-4071-7
Key Words Paenibacillus cucumis, polyphasic taxonomy, novel species, soil
Abstract Strain CO 4-7T was isolated from greenhouse soil used for cultivation of cucumbers in Korea. The 16S rRNA gene sequence of strain CO 4-7T showed the highest sequence similarity with Paenibacillus contaminans CKOBP-6T (94.2%) among the type strains. Strain CO 4-7T was a strictly aerobic, Gram-staining-positive, endospore-forming, and motile rodshaped bacterium. Strain CO 4-7T grew at 10–45°C (optimum, 30°C), at pH 6.0–7.5 (optimum, pH 6.5) and in the presence of 0–5% NaCl (optimum, 0.5%). The DNA G+C content of strain CO 4-7T was 48.5 mol%. It contained MK-7 as the major isoprenoid quinone and anteiso-C15:0 (51.8%), C16:0 (12.7%), and iso-C16:0 (8.6%) as the major fatty acids. The cell wall contained meso-diaminopimelic acid. Based on evidence from our polyphasic taxonomic study, it was concluded that strain CO 4-7T should be classified as a novel species of the genus Paenibacillus, for which, the name Paenibacillus cucumis sp. nov. is proposed. The type strain is CO 4-7T (=KACC 17444T=JCM 19515T).