| Title | Note] Identification of High-Specificity H-NS Binding Site in LEE5 Promoter of Enteropathogenic Esherichia coli (EPEC) |
|---|---|
| Author | Abhay Prasad Bhat1,2, Minsang Shin1,2, and Hyon E. Choy1,2* |
| Address | 1Center for Host Defense against Enteropathogenic Bacteria Infection, Chonnam National University Medical School, Kwangju 501-746, Republic of Korea, 2Department of Microbiology, Chonnam National University Medical School, Kwangju 501-746, Republic of Korea |
| Bibliography | Journal of Microbiology, 52(7),626-629, 2014, |
| DOI | 10.1007/s12275-014-3562-x |
| Key Words | LEE5, H-NS, nucleation site, consensus motif, transcriptional repression, gene regulation, transcription |
| Abstract | Histone-like nucleoid structuring protein (H-NS) is a small but abundant protein present in enteric bacteria and is involved in compaction of the DNA and regulation of the transcription. Recent reports have suggested that H-NS binds to a specific AT rich DNA sequence than to intrinsically curved DNA in sequence independent manner. We detected two high-specificity H-NS binding sites in LEE5 promoter of EPEC centered at -110 and -138, which were close to the proposed consensus H-NS binding motif. To identify H-NS binding sequence in LEE5 promoter, we took a random mutagenesis approach and found the mutations at around -138 were specifically defective in the regulation byH-NS. It was concluded that H-NS exertsmaximumrepression via the specific sequence at around -138 and ubsequently contacts α subunit of RNAP through oligomerization. |