Title |
Genotyping, Morphology and Molecular Characteristics of a Lytic Phage of Neisseria Strain Obtained from Infected Human Dental Plaque |
Author |
Ahmed N Aljarbou1* and Mohamad Aljofan1,2 |
Address |
1Department of Pharmaceutics, College of Pharmacy, Qassim University, Saudi Arabia, 2Department of Microbiology, School of Medicine and Health Sciences, Monash University, 3600, Australia |
Bibliography |
Journal of Microbiology, 52(7),609-618, 2014,
|
DOI |
10.1007/s12275-014-3380-1
|
Key Words |
Neisseria sp., Siphoviridae, lytic phage A2, human dental plaques, contigs, phylogenetic relationships |
Abstract |
The lytic bacteriaphage (phage) A2 was isolated from human dental plaques along with its bacterial host. The virus was found to have an icosahedron-shaped head (60±3 nm), a sheathed and rigid long tail (~175 nm) and was categorized into the family Siphoviridae of the order Caudovirales, which are dsDNA viral family, characterised by their ability to infect bacteria and are nonenveloped with a noncontractile tail. The isolated phage contained a linear dsDNA genome having 31,703 base pairs of unique sequence, which were sorted into three contigs and 12 single sequences. A latent period of 25 minutes and burst size of 24±2 particles was determined for the virus. Bioinformatics approaches were used to identify ORFs in the genome. A phylogenetic analysis confirmed the species inter-relationship and its placement in the family. |