| Title | Bacillus cheonanensis sp. nov. Isolated from Near Poultry Farm Soil |
|---|---|
| Author | Hyun-Ju Kim1, Cheol-Su Park2, Siwon Lee3, and Tae-Young Ahn1* |
| Address | 1Department of Microbiology, College of Natural Sciences, Dankook University, Cheonan 330-714, Republic of Korea, 2Plant Quarantine Technology Center, Animal and Plant Quarantine Agency, Suwon 443-440, Republic of Korea, 3Water Supply and Sewerage Research Division, National Institute of Environmental Research, Incheon 440-170, Republic of Korea |
| Bibliography | Journal of Microbiology, 52(7),554-558, 2014, |
| DOI | 10.1007/s12275-014-3458-9 |
| Key Words | poultry farm soil, Bacillus, polar lipid |
| Abstract | A novel bacterial strain, designated PFS-5T, was isolated from the soil environment with feces of a live poultry farm located in Cheonan, Republic of Korea. Strain PFS-5T was Gram-staining-positive, motile, strictly aerobic bacterium, rod-shaped, and endospore-forming. The strain contained meso-diaminopimelic acid in their peptidoglycan and MK-7 menaquinone. The major fatty acids were anteiso-C15:0 (44.2%), C16:0 (22.2%), and iso-C15:0 (16.7%). The DNA G+C content was 40.1 mol%. Comparative 16S rRNA gene sequence analysis identified strain PFS-5T in the genus Bacillus, exhibiting the highest level of sequence similarity with type strain of B. herbersteinensis D-1,5aT (96.9%), B. humi LMG 22167T (96.7%), B. alkalitelluris BA288T (96.1%), B. litoralis SW-211T (96.0%), and B. luteolus YIM93174T (95.5%). The major polar lipids of PFS-5T were diphosphatidylglycerol and phosphatidylglycerol. On the basis of result from poly-phasic data, strain PFS-5T represents a novel species, for which the name Bacillus cheonanensis sp. nov. is proposed (Type strain PFS-5T= KACC 17469T= JCM19333T). |