| Title | A Potent Brucella abortus 2308 Δery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection |
|---|---|
| Author | Junbo Zhang1, Shuanghong Yin2, Fei Guo2, Ren Meng1, Chuangfu Chen1*, Hui Zhang1, Zhiqiang Li1, Qiang Fu1, Huijun Shi1, Shengwei Hu1, Wei Ni3, Tiansen Li1, and Ke Zhang1 |
| Address | 1College of Animal Science and Technology, Shihezi University, Shihezi 832003, P. R. China, 2College of Medicine, Shihezi University, Shihezi 832003, P. R. China, 3College of Life Sciences, Shihezi University, Xinjiang 832000, P. R. China |
| Bibliography | Journal of Microbiology, 52(8),681–688, 2014, |
| DOI | 10.1007/s12275-014-3689-9 |
| Key Words | Brucella abortus 2308, ery promoter mutant, live attenuated vaccine, EryA |
| Abstract | Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (Δery) was constructed to overcome these drawbacks. The growth of the Δery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, Δery induced an anti-Brucellaspecific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-γ) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the Δery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection. |