| Title | Pyrosequencing-based Analysis of Fecal Microbial Communities in Three Purebred Pig Lines |
|---|---|
| Author | Edward Alain B. Pajarillo1, Jong Pyo Chae1, Marilen P. Balolong1,2, Hyeun Bum Kim1, Kang-Seok Seo3, and Dae-Kyung Kang1* |
| Address | 1Department of Animal Resources Science, Dankook University, Cheonan 330-714, Republic of Korea, 2Department of Biology, University of the Philippines Manila, Manila, 1000, Philippines, 3Department of Animal Science and Technology, Sunchon National University, Sunchon 540-950, Republic of Korea |
| Bibliography | Journal of Microbiology, 52(8),646–651, 2014, |
| DOI | 10.1007/s12275-014-4270-2 |
| Key Words | pyrosequencing, 16S rRNA genes, microbiome, pig breeds |
| Abstract | This study examined the fecal bacterial diversity of 15-week-old pigs from three purebred lines: Duroc, Landrace, and Yorkshire. Taxon-dependent and -independent analyses were performed to evaluate differences in the fecal bacterial communities and to identify bacterial genera that can be used to discriminate breeds, following high-throughput pyrosequencing of 16S rRNA genes. Among the breeds evaluated, Landrace had the most diverse bacterial community composition. Prevotella, Blautia, Oscillibacter, and Clostridium were detected in all samples regardless of breed. On the other hand, Catenibacterium, Blautia, Dialister, and Sphaerochaeta were differentially detected among breeds, as demonstrated by the canonical loading plot. The discriminant analysis of principal components plot also showed clear separation of the three purebred pig lines, with a certain degree of similarity between Landrace and Yorkshire pigs and a distinct separation between Duroc pigs and the other two breeds. Other factors not related to breed, such as season or time of sampling and pen effects, may contribute to shaping the gut microbiota of pigs. |