| Title | Note] Analysis of a draft genome sequence of Kitasatospora cheerisanensis KCTC 2395 producing bafilomycin antibiotics |
|---|---|
| Author | Jae Yoon Hwang1, Soo Hee Kim1, Hye Ryeung Oh1, Eunju Kwon2, and Doo Hyun Nam1* |
| Address | 1College of Pharmacy, Yeungnam University, Gyongsan 712-749, Republic of Korea, 2Institute for Drug Development, Yeungnam University, Gyongsan 712-749, Republic of Korea |
| Bibliography | Journal of Microbiology, 53(1),84-89, 2015, |
| DOI | 10.1007/s12275-015-4340-0 |
| Key Words | draft genome, Kitasatospora, MurE, polyketide synthase, bafilomycin |
| Abstract | Kitasatospora cheerisanensis KCTC 2395, producing bafilomycin antibiotics belonging to plecomacrolide group, was isolated from a soil sample at Mt. Jiri, Korea. The draft genome sequence contains 8.04 Mb with 73.6% G+C content and 7,810 open reading frames. All the genes for aerial mycelium and spore formations were confirmed in this draft genome. In phylogenetic analysis of MurE proteins (UDPN- acetylmuramyl-L-alanyl-D-glutamate:DAP ligase) in a conserved dcw (division of cell wall) locus, MurE proteins of Kitasatospora species were placed in a separate clade between MurEs of Streptomyces species incorporating LL-diaminopimelic acid (DAP) and MurEs of Saccharopolyspora erythraea as well as Mycobacterium tuberculosis ligating meso- DAP. From this finding, it was assumed that Kitasatospora MurEs exhibit the substrate specificity for both LL-DAP and meso-DAP. The bafilomycin biosynthetic gene cluster was located in the left subtelomeric region. In 71.3 kb-long gene cluster, 17 genes probably involved in the biosynthesis of bafilomycin derivatives were deduced, including 5 polyketide synthase (PKS) genes comprised of 12 PKS modules. |