Title |
Performance of a real-time PCR assay for the rapid identification of Mycobacterium species |
Author |
Hye-young Wang1, Hyunjung Kim2, Sunghyun Kim2,3, Do-kyoon Kim4, Sang-Nae Cho5, and Hyeyoung Lee2* |
Address |
1M&D, Inc., Wonju Eco Environmental Technology Center, Wonju 200-722, Republic of Korea, 2Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju 220-710, Republic of Korea, 3Institute for Life Science and Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea, 4Department of Laboratory Medicine, The Catholic University of Korea, St. Vincent’s Hospital, Suwon 442-723, Republic of Korea, 5Department of Microbiology, College of Medicine, Yonsei University, Seoul 120-749, Republic of Korea |
Bibliography |
Journal of Microbiology, 53(1),38-46, 2015,
|
DOI |
10.1007/s12275-015-4495-8
|
Key Words |
Mycobacterium tuberculosis (MTB), nontuberculous
mycobacteria (NTM), Real Myco-ID?, identification,
molecular diagnosis |
Abstract |
Mycobacteria cause a variety of illnesses that differ in severity
and public health implications. The differentiation of
Mycobacterium tuberculosis (MTB) from nontuberculous
mycobacteria (NTM) is of primary importance for infection
control and choice of antimicrobial therapy. The diagnosis
of diseases caused by NTM is difficult because NTM species
are prevalent in the environment and because they have fastidious
properties. In the present study, we evaluated 279
clinical isolates grown in liquid culture provided by The
Catholic University of Korea, St. Vincent’s Hospital using
real-time PCR based on mycobacterial rpoB gene sequences.
The positive rate of real-time PCR assay accurately discriminated
100% (195/195) and 100% (84/84) between MTB and
NTM species. Comparison of isolates identified using the
MolecuTech REBA Myco-ID? and Real Myco-ID? were completely
concordant except for two samples. Two cases that
were identified as mixed infection (M. intracellulare-M. massiliense
and M. avium-M. massiliense co-infection) by PCRREBA
assay were only detected using M. abscessus-specific
probes by Real Myco-ID?. Among a total of 84 cases, the
most frequently identified NTM species were M. intracellulare
(n=38, 45.2%), M. avium (n=18, 23.7%), M. massiliense
(n=10, 13.2%), M. fortuitum (n=5, 6%), M. abscessus
(n=3, 3.9%), M. gordonae (n=3, 3.9%), M. kansasii (n=2,
2.4%), M. mucogenicum (n=2, 2.4%), and M. chelonae (n=
1, 1.2%). Real Myco-ID? is an efficient tool for the rapid detection
of NTM species as well as MTB and sensitive and
specific and comparable to conventional methods. |