| Title | Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting |
|---|---|
| Author | Daisy Pérez-Brito1, Anuar Magaña-Alvarez1, Patricia Lappe-Oliveras2, Alberto Cortes-Velazquez1, Claudia Torres-Calzada1, Teófilo Herrera-Suarez2, Alfonso Larqué-Saavedra3, and Raul Tapia-Tussell1* |
| Address | 1Laboratorio GeMBio, Centro de Investigación Científica de Yucatán A.C., Calle 43 # 130, Col. Chuburná de Hidalgo, Mérida, Yucatán 97200, México, 2Instituto de Biología, Universidad Nacional Autónoma de México, 3er Circuito S/N, Ciudad Universitaria, Del. Coyoacán, México DF. 04510, México, 3Centro de Investigación Científica de Yucatán A.C., Calle 43 # 130, Col. Chuburná de Hidalgo, Mérida, Yucatán 97200, México |
| Bibliography | Journal of Microbiology, 53(1),14-20, 2015, |
| DOI | 10.1007/s12275-015-4373-4 |
| Key Words | Agave fourcroydes, Clavispora lusitaniae, D1/D2 large-subunit rDNA polymorphism, DNA fingerprinting, genetic diversity |
| Abstract | This study characterized Clavispora lusitaniae strains isolated from different stages of the processing and early fermentation of a henequen (Agave fourcroydes) spirit produced in Yucatan, Mexico using a molecular technique. Sixteen strains identified based on morphological features, obtained from different substrates, were typed molecularly. Nine different versions of the divergent D1/D2 domain of the large-subunit ribosomal DNA sequence were identified among the C. lusitaniae strains. The greatest degree of polymorphism was found in the 90-bp structural motif of the D2 domain. The MSP-PCR technique was able to differentiate 100% of the isolates. This study provides significant insight into the genetic diversity of the mycobiota present during the henequen fermentation process, especially that of C. lusitaniae, for which only a few studies in plants have been published. The applied MSP-PCR markers were very efficient in revealing polymorphisms between isolates of this species. |