Title |
Description of Pseudomonas asuensis sp. nov. from biological soil crusts in the Colorado plateau, United States of America |
Author |
Gundlapally Sathyanarayana Reddy*,# and Ferran Garcia-Pichel |
Address |
School of Life Sciences, Arizona State University, Main Campus, Tempe, AZ 85287-4501, USA, #Present address: CSIR-Centre for Cellular and Molecular Biology Uppal Road, Hyderabad 500 007, India |
Bibliography |
Journal of Microbiology, 53(1),6-13, 2015,
|
DOI |
10.1007/s12275-015-4462-4
|
Key Words |
Pseudomonas asuensis, biological soil crusts,
Colorado plateau, United States of America |
Abstract |
A Gram-negative, aerobic, non spore-forming, non-motile,
rod-shaped, yellow pigmented bacterium CP155-2T was isolated
from a biological soil crusts sample collected in the
Colorado plateau, USA and subjected to polyphasic taxonomic
characterization. Strain CP155-2T contained summed
feature 3 (C16:1ω5c/C16:1ω7c) and C18:1ω7c as major fatty
acids and diphosphatidylglycerol (DPG) along with phosphatidylethanolamine
(PE) and phosphatidylglycerol (PG)
as major polar lipids. Based on these characteristics CP155-2T
was assigned to the genus Pseudomonas. Phylogenetic analysis
based on 16S rRNA gene sequence further confirmed the
affiliation of CP155-2T to the genus Pseudomonas and showed
a 16S rRNA gene sequence similarity of less than 98.7% with
already described species of the genus. Pseudomonas luteola,
Pseudomonas zeshuii, and Pseudomonas duriflava were identified
as the closest species of the genus Pseudomonas with
16S rRNA gene sequence similarities of 98.7%, 98.6%, and
96.9%, respectively. The values for DNA–DNA relatedness
between CP155-2T and Pseudomonas luteola and Pseudomonas
zeshuii were 23% and 14% respectively a value below
the 70% threshold value, indicating that strain CP155-2T
belongs to a novel taxon of the genus Pseudomonas lineage.
The novel taxon status was strengthened by a number of phenotypic
differences wherein CP155-2T was positive for oxidase,
negative for gelatin hydrolysis, could utilize D-cellobiose,
D-raffinose, L-rhamnose, D-sorbitol but not L-aspartic
acid and L-glutamic acid. Based on the collective differences
strain CP155-2T exhibited, it was identified as a novel species
and the name Pseudomonas asuensis sp. nov. was proposed.
The type strain of Pseudomonas asuensis sp. nov. is CP155-
2T (DSM 17866T =ATCC BAA-1264T =JCM13501T =KCTC
32484T). |