Title |
Effects of Lactobacillus salivarius Ren on cancer prevention and intestinal microbiota in 1, 2-dimethylhydrazine-induced rat model |
Author |
Ming Zhang1,2, Xing Fan3,4, Bing Fang5, Chengzhen Zhu3,4, Jun Zhu3,4, and Fazheng Ren3,4* |
Address |
1School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, P. R. China, 2Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048, China, 3Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P. R. China, 4Beijing Laboratory for Food Quality and Safety, China Agricultural University, Beijing 100083, P. R. China, 5Academy of State Administration of Grain, Beijing 100037, P. R. China |
Bibliography |
Journal of Microbiology, 53(6),398-405, 2015,
|
DOI |
10.1007/s12275-015-5046-z
|
Key Words |
colorectal cancer, microbiota, Lactobacillus salivarius
Ren, DMH |
Abstract |
Probiotics have been suggested as a prophylactic measure
in colon cancer. The aim of this study was to investigate the
impact of Lactobacillus salivarius Ren (Ren) in modulating
colonic microbiota structure and colon cancer incidence in a
rat model after injection with 1,2-dimethyl hydrazine (DMH).
The results indicated that oral administration of Ren could
effectively suppress DMH-induced colonic carcinogenesis.
A significant decrease in cancer incidence (87.5% to 25%)
was detected in rats fed with a dose of 5 × 1010 CFU/kg
bodyweight per day. Using denaturing gradient gel electrophoresis
and Real-time PCR combined with multivariate
statistical methods, we demonstrated that injection with DMH
significantly altered the rat gut microbiota, while Ren counteracted
these DMH-induced adverse effects and promoted
reversion of the gut microbiota close to the healthy state. Tvalue
biplots followed by band sequencing identified 21 bacterial
strains as critical variables affected by DMH and Ren.
Injection of DMH significantly increased the amount of
Ruminococcus species (sp.) and Clostridiales bacteria, as well
as decreasing the Prevotella sp. Administration of Ren reduced
the amount of Ruminococcus sp., Clostridiales bacteria, and
Bacteroides dorei, and increased the amount of Prevotella.
Real-time PCR results were consistent with the results derived
by t-value biplots. These findings suggested that Ren is a potential
agent for colon cancer prevention. In conclusion, the
results in the present study suggest a potential therapeutic
approach based on the modulation of intestinal microflora
by probiotics may be beneficial in the prevention of colorectal
carcinogenesis. |