Title |
An endophytic Coniochaeta velutina producing broad spectrum antimycotics |
Author |
Jie Xie1, Gary A. Strobel2*, Tao Feng3, Huishuang Ren1, Morgan T. Mends2, Zeyang Zhou1, and Brad Geary4 |
Address |
1State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, P. R. China, 2Department of Plant Sciences, Montana State University, Bozeman, Montana, 59717, USA, 3State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, P. R. China, 4Department of Plant and Wildlife Sciences, Brigham Young University, Provo, UT 84602, USA |
Bibliography |
Journal of Microbiology, 53(6),390-397, 2015,
|
DOI |
10.1007/s12275-015-5105-5
|
Key Words |
antimycotics, endophyte, Coniochaeta velutina,
bioassays, SEM |
Abstract |
An endophyte (PC27-5) was isolated from stem tissue of
Western hemlock (Tsuga heterophylla) in a Pacific Northwest
temperate rainforest. Phylogenetic analyses, based on ITS-
5.8S rDNA and 18S rDNA sequence data, combined with
cultural and morphological analysis showed that endophyte
PC27-5 exhibited all characteristics of a fungus identical to
Coniochaeta velutina. Furthermore, wide spectrum antimycotics
were produced by this endophyte that were active
against such plant pathogens as Sclerotinia sclerotiorum, Pythium
ultimum, and Verticillium dahliae and lethal to Phythophthora
cinnamomi, Pythium ultimum, and Phytophthora
palmivora in plate tests. The bioactive components were purified
through organic solvent extraction, followed by silica
column chromatography, and finally preparative HPLC. The
minimum inhibitory concentration of the active fraction to
Pythium ultimum, which was gained from preparative HPLC,
was 11 ?/ml. UPLC-HRMS analysis showed there were two
similar components in the antimycotic fraction. Their molecular
formulae were established as C30H22O11 (compound I)
and C30H22O10 (compound II) respectively, and preliminary
spectral results indicate that they are anthroquinone glycosides.
Other non ?biologically active compounds were identified
in culture fluids of this fungus by spectral means as emodin
and chrysophanol - anthroquinone derivatives. This is
the first report that Coniochaeta velutina as an endophyte
produces bioactive antifungal components. |