Title |
Microbial communities inhabiting oil-contaminated soils from two major oilfields in Northern China: Implications for active petroleum-degrading capacity |
Author |
Weimin Sun1, Yiran Dong2, Pin Gao3, Meiyan Fu4, Kaiwen Ta5, and Jiwei Li5* |
Address |
1Department of Microbiology and Biochemistry, Rutgers University, New Brunswick, NJ, 08901, USA, 2Energy and Bioscience Institute, University of Illinois-Urbana Champaign, Urbana, IL, 61801, USA, 3School of Environmental Science and Engineering, Donghua University, 201620, Shanghai, P. R. China, 4College of Energy Resources, Chengdu University of Technology, Chengdu, 610059, P. R. China, 5Sanya Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, 572000, P. R. China |
Bibliography |
Journal of Microbiology, 53(6),371-378, 2015,
|
DOI |
10.1007/s12275-015-5023-6
|
Key Words |
oil-degrading bacteria, bioremediation, microbial
community analysis, Illumina sequencing |
Abstract |
Although oilfields harbor a wide diversity of microorganisms
with various metabolic potentials, our current knowledge
about oil-degrading bacteria is limited because the vast majority
of oil-degrading bacteria remain uncultured. In the
present study, microbial communities in nine oil-contaminated
soils collected from Daqing and Changqing, two of the
largest oil fields in China, were characterized through highthroughput
sequencing of 16S rRNA genes. Bacteria related
to the phyla Proteobacteria and Actinobacteria were dominant
in four and three samples, respectively. At the genus level,
Alkanindiges, Arthrobacter, Pseudomonas, Mycobacterium,
and Rhodococcus were frequently detected in nine soil samples.
Many of the dominant genera were phylogenetically
related to the known oil-degrading species. The correlation
between physiochemical parameters within the microbial
communities was also investigated. Canonical correspondence
analysis revealed that soil moisture, nitrate, TOC, and pH
had an important impact in shaping the microbial communities
of the hydrocarbon-contaminated soil. This study provided
an in-depth analysis of microbial communities in oilcontaminated
soil and useful information for future bioremediation
of oil contamination. |