Title |
Lysobacter tyrosinelyticus sp. nov. isolated from Gyeryongsan national park soil |
Author |
Juan Du1, Hina Singh1, Hien T.T. Ngo1, KyungHwa Won1, Ki-Young Kim2, and Tae-Hoo Yi1* |
Address |
1Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University Global Campus, Gyeonggi-do 446-701, Republic of Korea, 2Department of Genetic Engineering, College of Life Science, Kyung Hee University Global Campus, Gyeonggi-do 446-701, Republic of Korea |
Bibliography |
Journal of Microbiology, 53(6),365-370, 2015,
|
DOI |
10.1007/s12275-015-4729-9
|
Key Words |
Lysobacter tyrosinelyticus, Gram-negative, Ubiquinone
Q-8, 16S rRNA |
Abstract |
A novel Gram-negative, rod-shaped (0.2-0.5 um x 1.5-2.5 um), aerobic, non-motile bacterium was isolated from Gyeryongsan
national park soil, Republic of Korea. The novel
isolate was designated as THG-DN8.2T. The strain grows
optimally at 28oC, at pH 7 and in the absence of NaCl.
Phylogenetic analysis based on 16S rRNA gene sequence
showed that the novel isolate shared the highest sequence
similarity with Lysobacter oryzae KCTC 22249T followed by
Lysobacter yangpyeongensis KACC 11407T and Lysobacter
niabensis KACC 11587T. The DNA G+C content of strain
THG-DN8.2T is 66.0 mol% and ubiquinone Q-8 is the main
isoprenoid quinone. The major polar lipids were diphosphatidylglycerol,
phosphatidylglycerol, phosphatidylethanolamine,
and phosphatidyl-N-methylethanolamine. The
major fatty acids of strain THG-DN8.2T were identified as
iso-C15:0, iso-C16:0, and C16:1w7c alcohol. The phylogenetic
distinctiveness and phenotypic characteristics differentiated
strain THG-DN8.2T from closely related Lysobacter species.
The results of polyphasic taxonomic analysis suggest that
strain THG-DN8.2T represents a novel species of the genus
Lysobacter, for which the name Lysobacter tyrosinelyticus
sp. nov. is proposed. The type strain is THG-DN8.2T (=KCTC
42235T =JCM 30320T). |