Title Spirosoma montaniterrae sp. nov., an ultraviolet and gamma radiation-resistant bacterium isolated from mountain soil
Author Jae-Jin Lee1, Myung-Suk Kang2, Eun Sun Joo1, Myung Kyum Kim1, Wan-Taek Im3, Hee-Young Jung4, and Sathiyaraj Srinivasan1*
Address 1Department of Bio & Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul 139-774, Republic of Korea, 2Microorganism Resources Division, National Institute of Biological Resources, Incheon 404-170, Republic of Korea, 3Department of Biotechnology, Hankyong National Univeristy, Kyonggi-do 456-749, Republic of Korea, 4College of Agricultural and Life Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea
Bibliography Journal of Microbiology, 53(7),429-434, 2015,
DOI 10.1007/s12275-015-5008-5
Key Words Cytophagaceae, Spirosoma montaniterrae, radiation-resistant, taxonomy
Abstract A Gram-negative, yellow-pigmented, long-rod shaped bacterial strain designated DY10T was isolated from a soil sample collected at Mt. Deogyusan, Jeonbuk province, South Korea. Optimum growth observed at 30°C and pH 7. No growth was observed above 1% (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain DY10T belonged to the genus Spirosoma and was distantly related to Spirosoma arcticum R2-35T (91.0%), Spirosoma lingual DSM 74T (90.8%), Spirosoma endophyticum EX36T (90.7%), Spirosoma panaciterrae DSM 21099T (90.5%), Spirosoma rigui WPCB118T (90.2%), Spirosoma spitsbergense DSM 19989T (89.8%), Spirosoma luteum DSM 19990T (89.6%), Spirosoma oryzae RHs22T (89.6%), and Spirosoma radiotolerans DG5AT (89.1%). Strain DY10T showed resistance to gamma and ultraviolet radiation. The chemotaxonomic characteristics of strain DY10T were consistent with those of the genus Spirosoma, with the quinone system with MK-7 as the predominant menaquinone, iso-C15:0, C16:1 ω5c, and summed feature3 (C16:1 ω7c/C16:1 ω6c), and phosphatidylethanolamine as the major polar lipid. The G+C content of the genomic DNA was 53.0 mol%. Differential phenotypic properties with the closely related type strains clearly distinguished strain DY10T from previously described members of the genus Spirosoma and represents a novel species in this genus, for which the name Spirosoma montaniterrae sp. nov. is proposed. The type strain is DY10T (=KCTC 23999T =KEMB 9004-162T =JCM 18492T).