Title |
Description of a novel pectin-degrading bacterial species Prevotella pectinovora sp. nov., based on its phenotypic and genomic traits |
Author |
Brigita Nograsek, Tomaz Accetto, Lijana Fanedl, and Gorazd Avgustin* |
Address |
University of Ljubljana, Biotechnical Faculty, Animal Science Department, Division of Microbiology and Microbial Biotechnology, Groblje 3, 1230 Domzale, Slovenia |
Bibliography |
Journal of Microbiology, 53(8),503-510, 2015,
|
DOI |
10.1007/s12275-015-5142-0
|
Key Words |
anaerobic bacterium, Prevotella pectinovora sp.
nov., pectin degradation and utilization, genome analysis,
pig feces, taxonomy |
Abstract |
Five strictly anaerobic Gram-negative bacterial strains, P4-65,
P4-76T, P5-60, P5-119, and P5-125, presumably belonging
to the genus Prevotella were isolated from pig fecal samples.
Strains were tested for various phenotypic traits and nearcomplete
genome sequences were obtained and analyzed.
Phylogenetic analysis based on 16S rRNA gene sequences and
multilocus sequence analysis based on five conserved genes
confirmed that the strains belong to the genus Prevotella,
revealing that they represent a novel and discrete lineage
distinct from other known species of this genus. The size of
the genome of the isolated strains is 3?.3 Mbp, and the
DNA G+C content is 47.5?8.1 mol%. The isolates are strictly
anaerobic, rod-shaped with rounded ends, non-motile and
non-spore-forming. The main fermentation products are
succinate and acetate, with minor concentrations of isovalerate,
propionate and isobutyrate. Hydrogen is also produced.
Major cellular fatty acids consist of anteiso-C15:0 and iso-C15:0,
and a number of additional acids are present in lower concentrations.
A substantial portion of genes involved in carbohydrate
utilization is devoted to pectin degradation and utilization,
while those supporting growth on xylan in ruminal
Prevotella could not have been revealed. On the basis of the
presented results, a novel species, Prevotella pectinovora sp.
nov. is proposed. The type strain is P4-76T (=DSM 29996T
=ZIM B1020T). |