Title |
The hrp pathogenicity island of Pseudomonas syringae pv. tomato DC3000 is induced by plant phenolic acids |
Author |
Jun Seung Lee1, Hye Ryun Ryu2, Ji Young Cha3, and Hyung Suk Baik1* |
Address |
1Department of Integrated Biological Science, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea, 2R & D, CJ Cheil Jedang Corporation, Seoul 04560, Republic of Korea, 3Department of Biotechnology and Bioengineering, College of Engineering, Dong-Eui University, Busan 47340, Republic of Korea |
Bibliography |
Journal of Microbiology, 53(10),725-731, 2015,
|
DOI |
10.1007/s12275-015-5256-4
|
Key Words |
plant phenolic compounds, Pseudomonas syringae,
plant-pathogen interaction, hrp PAI, qRT-PCR |
Abstract |
Plants produce a wide array of antimicrobial compounds,
such as phenolic compounds, to combat microbial pathogens.
The hrp PAI is one of the major virulence factors in the
plant pathogen, Pseudomonas syringae. A major role of hrp
PAI is to disable the plant defense system during bacterial
invasion. We examined the influence of phenolic compounds
on hrp PAI gene expression at low and high concentrations.
There was approximately 2.5 times more hrpA and hrpZ
mRNA in PtoDC3000 that was grown in minimal media
(MM) supplemented with 10 μM of ortho-coumaric acid than
in PtoDC3000 grown in MM alone. On the other hand, a
significantly lower amount of hrpA mRNA was observed in
bacteria grown in MM supplemented with a high concentration
of phenolic compounds. To determine the regulation
pathway for hrp PAI gene expression, we performed qRTPCR
using gacS, gacA, and hrpS deletion mutants. |