Title The hrp pathogenicity island of Pseudomonas syringae pv. tomato DC3000 is induced by plant phenolic acids
Author Jun Seung Lee1, Hye Ryun Ryu2, Ji Young Cha3, and Hyung Suk Baik1*
Address 1Department of Integrated Biological Science, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea, 2R & D, CJ Cheil Jedang Corporation, Seoul 04560, Republic of Korea, 3Department of Biotechnology and Bioengineering, College of Engineering, Dong-Eui University, Busan 47340, Republic of Korea
Bibliography Journal of Microbiology, 53(10),725-731, 2015,
DOI 10.1007/s12275-015-5256-4
Key Words plant phenolic compounds, Pseudomonas syringae, plant-pathogen interaction, hrp PAI, qRT-PCR
Abstract Plants produce a wide array of antimicrobial compounds, such as phenolic compounds, to combat microbial pathogens. The hrp PAI is one of the major virulence factors in the plant pathogen, Pseudomonas syringae. A major role of hrp PAI is to disable the plant defense system during bacterial invasion. We examined the influence of phenolic compounds on hrp PAI gene expression at low and high concentrations. There was approximately 2.5 times more hrpA and hrpZ mRNA in PtoDC3000 that was grown in minimal media (MM) supplemented with 10 μM of ortho-coumaric acid than in PtoDC3000 grown in MM alone. On the other hand, a significantly lower amount of hrpA mRNA was observed in bacteria grown in MM supplemented with a high concentration of phenolic compounds. To determine the regulation pathway for hrp PAI gene expression, we performed qRTPCR using gacS, gacA, and hrpS deletion mutants.