Title |
Relationships between the use of Embden Meyerhof pathway (EMP) or Phosphoketolase pathway (PKP) and lactate production capabilities of diverse Lactobacillus reuteri strains |
Author |
Grégoire Burgé1,2,3, Claire Saulou-Bérion2,3, Marwen Moussa2,3, Florent Allais1,2,3, Violaine Athes2,3, and Henry-Eric Spinnler2,3* |
Address |
1Chaire Agro-Biotechnologies Industrielles (ABI) - AgroParisTech, 247 rue Paul Vaillant Couturier, F-51100 Reims, France, 2AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850 Thiverval-Grignon, France, 3INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850 Thiverval-Grignon, France |
Bibliography |
Journal of Microbiology, 53(10),702-710, 2015,
|
DOI |
10.1007/s12275-015-5056-x
|
Key Words |
Lactobacillus reuteri, microbial growth, acidification
kinetics, glucose metabolism, Embden-Meyerhof pathway,
Phosphoketolase pathway, lactate production |
Abstract |
The aims of this study is to compare the growth and glucose
metabolism of three Lactobacillus reuteri strains (i.e.
DSM 20016, DSM 17938, and ATCC 53608) which are lactic
acid bacteria of interest used for diverse applications such
as probiotics implying the production of biomass, or for the
production of valuable chemicals (3-hydroxypropionaldehyde,
3-hydroxypropionic acid, 1,3-propanediol). However, the
physiological diversity inside the species, even for basic metabolisms,
like its capacity of acidification or glucose metabolism,
has not been studied yet. In the present work, the
growth and metabolism of three strains representative of
the species diversity have been studied in batch mode. The
strains were compared through characterization of growth
kinetics and evaluation of acidification kinetics, substrate consumption
and product formation. The results showed significant
differences between the three strains which may be
explained, at least in part, by variations in the distribution
of carbon source between two glycolytic pathways during the
bacterial growth: the phosphoketolase or heterolactic pathway
(PKP) and the Embden-Meyerhof pathway (EMP). It was
also shown that, in the context of obtaining a large amount
of biomass, DSM 20016 and DSM 17938 strains were the
most effective in terms of growth kinetics. The DSM 17938
strain, which shows the more significant metabolic shift from
EMP to PKP when the pH decreases, is more effective for
lactate production. |