Title Sphingomonas parvus sp. nov. isolated from a ginseng-cultivated soil
Author Jae-Hyung Ahn1, Byoung-chan Kim2, Soo-Jin Kim1, Geun-Hey Lee1, Jaekyeong Song1, Soon-Wo Kwon1, and Hang-Yeon Weon1*
Address 1Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea, 2Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea
Bibliography Journal of Microbiology, 53(10),673-677, 2015,
DOI 10.1007/s12275-015-5132-2
Key Words Sphingomonas, strain GP20-2, novel species, polyphasic taxonomy
Abstract Strain GP20-2T was isolated from a soil cultivated with ginseng in Korea. The 16S rRNA gene sequence of this strain showed the highest sequence similarity with Sphingomonas daechungensis CH15-11T (96.7%) and Sphingomonas sediminicola Dae 20T (96.2%) among the type strains. The strain GP20-2T was a strictly aerobic, Gram-negative, non-motile, rod-shaped bacterium that formed very tiny colonies, less than 0.3 mm in diameter after 10 days on R2A agar. The strain grew at 10–35°C (optimum, 35°C), at a pH of 5.0–8.0 (optimum, pH 6.0), and in the absence of NaCl. The DNA G+C content of strain GP20-2T was 67.2 mol%. It contained ubiquinone Q-10 as the major isoprenoid quinone, and summed feature 8 (C18:1ω6c and/or C18:1ω7c, 49.8%) and C16:0 (17.0%) as the major fatty acids. On the basis of evidence from our polyphasic taxonomic study, we concluded that strain GP20-2T should be classified as a novel species of the genus Sphingomonas, for which the name Sphingomonas parvus sp. nov. is proposed. The type strain is GP20-2T (=KACC 12865T =DSM 100456T).