Title |
Inhibition of quorum sensing, biofilm, and spoilage potential in Shewanella baltica by green tea polyphenols |
Author |
Junli Zhu1*, Xuzheng Huang1, Fang Zhang1, Lifang Feng1, and Jianrong Li2 |
Address |
1College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, P. R. China, 2College of Chemistry, Chemical Engineering and Food Safety, Bohai University, Food Safety Key Lab of Liaoning Province, Jinzhou, 121013, P. R. China |
Bibliography |
Journal of Microbiology, 53(12),829-836, 2015,
|
DOI |
10.1007/s12275-015-5123-3
|
Key Words |
Shewanella baltica, AI-2, diketopiperazines, green
tea polyphenols, biofilm, fish spoilage |
Abstract |
We investigated the quorum sensing (QS) system of Shewanella
baltica and the anti-QS related activities of green tea
polyphenols (TP) against spoilage bacteria in refrigerated large
yellow croaker. Autoinducer-2 (AI-2) and the diketopiperazines
(DKPs) cyclo-(L-Pro-L-Leu) and cyclo-(L-Pro-L-Phe) were
detected in the culture extract of S. baltica XH2, however,
no N-acylhomoserine lactones (AHLs) activity was observed.
Green TP at sub-inhibitory concentrations interfered with
AI-2 and DKPs activities of S. baltica without inhibiting
cell growth and promoted degradation of AI-2. The green TP
treatment inhibited biofilm development, exopolysaccharide
production and swimming motility of S. baltica in a concentration-
dependent manner. In addition, green TP decreased
extracellular protease activities and trimethylamine production
in S. baltica. A transcriptional analysis showed that green
TP repressed the luxS and torA genes in S. baltica, which
agreed with the observed reductions in QS activity and the
spoilage phenotype. Epigallocatechin gallate (EGCG)-enriched
in green TP significantly inhibited AI-2 activity of S.baltica.
These findings strongly suggest that green TP could be developed
as a new QS inhibitor for seafood preservation to
enhance shelf life. |