Title |
Interferon-mediated antiviral activities of Angelica tenuissima Nakai and its active components |
Author |
Prasanna Weeratunga1, Md Bashir Uddin1,2, Myun Soo Kim3, Byeong-Hoon Lee1, Tae-Hwan Kim1, Ji-Eun Yoon4, Jin Yeul Ma5, Hongik Kim3, and Jong-Soo Lee1* |
Address |
1College of Veterinary Medicine (BK21 Plus Program), Chungnam National University, Daejeon 305-764, Republic of Korea, 2Faculty of Veterinary & Animal Science, Sylhet Agricultural University, Sylhet-3100, Bangladesh, 3Vitabio Corporation, Daejeon 305-764, Republic of Korea, 4Foot and Mouth Disease Division, Animal Quarantine and Inspection Agency, Anyang, Republic of Korea, 5Korean Medicine (KM) Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, Daejeon 305-764, Republic of Korea |
Bibliography |
Journal of Microbiology, 54(1),57-70, 2016,
|
DOI |
10.1007/s12275-016-5555-4
|
Key Words |
Angelica tenuissima Nakai, ferulic acid, herbal
medicine, anti-influenza effect, antiviral effect |
Abstract |
Angelica tenuissima Nakai is a widely used commodity in
traditional medicine. Nevertheless, no study has been conducted
on the antiviral and immune-modulatory properties
of an aqueous extract of Angelica tenuissima Nakai. In the
present study, we evaluated the antiviral activities and the
mechanism of action of an aqueous extract of Angelica tenuissima
Nakai both in vitro and in vivo. In vitro, an effective
dose of Angelica tenuissima Nakai markedly inhibited the
replication of Influenza A virus (PR8), Vesicular stomatitis
virus (VSV), Herpes simplex virus (HSV), Coxsackie virus,
and Enterovirus (EV-71) on epithelial (HEK293T/HeLa) and
immune (RAW264.7) cells. Such inhibition can be described
by the induction of the antiviral state in cells by antiviral, IFNrelated
gene induction and secretion of IFNs and pro-inflammatory
cytokines. In vivo, Angelica tenuissima Nakai
treated BALB/c mice displayed higher survivability and lower
lung viral titers when challenged with lethal doses of highly
pathogenic influenza A subtypes (H1N1, H5N2, H7N3, and
H9N2). We also found that Angelica tenuissima Nakai can
induce the secretion of IL-6, IFN-λ, and local IgA in bronchoalveolar
lavage fluid (BALF) of Angelica tenuissima Nakai
treated mice, which correlating with the observed prophylactic
effects. In HPLC analysis, we found the presence of several
compounds in the aqueous fraction and among them; we
evaluated antiviral properties of ferulic acid. Therefore, an
extract of Angelica tenuissima Nakai and its components,
including ferulic acid, play roles as immunomodulators and
may be potential candidates for novel anti-viral/anti-influenza
agents. |