| Title | TatC-dependent translocation of pyoverdine is responsible for the microbial growth suppression |
|---|---|
| Author | Yeji Lee1, Yong-Jae Kim1, Jung-Hoon Lee1, Hyung Eun Yu2,3, Kiho Lee2, Shouguang Jin4, and Un-Hwan Ha1* |
| Address | 1Department of Biotechnology and Bioinformatics, Korea University, Sejong 339-700, Republic of Korea, 2Department of Pharmacy, Korea University, Sejong 339-700, Republic of Korea, 3New Drug Discovery Lab, Hyundai Pharmaceutical Co., Ltd., Yongin 449-060, Republic of Korea, 4Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, USA |
| Bibliography | Journal of Microbiology, 54(2),122-130, 2016, |
| DOI | 10.1007/s12275-016-5542-9 |
| Key Words | growth suppression, pyoverdine, TatC |
| Abstract | Infections are often not caused by a colonization of Pseudomonas aeruginosa alone but by a consortium of other bacteria. Little is known about the impact of P. aeruginosa on the growth of other bacteria upon coinfection. Here, cellree culture supernatants obtained from P. aeruginosa suppressed the growth of a number of bacterial strains such as Corynebacterium glutamicum, Bacillus subtilis, Staphylococcus aureus, and Agrobacterium tumefaciens, but had little effect on the growth of Escherichia coli and Salmonella Typhimurium. The growth suppression effect was obvious when P. aeruginosa was cultivated in M9 minimal media, and the suppression was not due to pyocyanin, a well-known antimicrobial toxin secreted by P. aeruginosa. By performing transposon mutagenesis, PA5070 encoding TatC was identified, and the culture supernatant of its mutant did not suppress the growth. HPLC analysis of supernatants showed that pyoverdine was a secondary metabolite present in culture supernatants of the wild-type strain, but not in those of the PA5070 mutant. Supplementation of FeCl2 as a source of iron compromised the growth suppression effect of supernatants and also recovered biofilm formation of S. aureus, indicating that pyoverdine-mediated iron acquisition is responsible for the growth suppression. Thus, this study provides the action of TatC-dependent pyoverdine translocation for the growth suppression of other bacteria, and it might aid understanding of the impact of P. aeruginosa in the complex community of bacterial species upon coinfection. |