Title |
Effects of blue light on pigment biosynthesis of Monascus |
Author |
Di Chen, Chunmao Xue, Mianhua Chen, Shufen Wu, Zhenjing Li, and Changlu Wang* |
Address |
Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, TEDA, Tianjin, 300457, P. R. China |
Bibliography |
Journal of Microbiology, 54(4),305-310, 2016,
|
DOI |
10.1007/s12275-016-6011-1
|
Key Words |
Monascus, blue light, illumination level, pigments,
biosynthetic genes |
Abstract |
The influence of different illumination levels of blue light
on the growth and intracellular pigment yields of Monascus
strain M9 was investigated. Compared with darkness, constant
exposure to blue light of 100 lux reduced the yields of six
pigments, namely, rubropunctatamine (RUM), monascorubramine
(MOM), rubropunctatin (RUN), monascorubrin
(MON), monascin (MS), and ankaflavin (AK). However,
exposure to varying levels of blue light had different effects
on pigment production. Exposure to 100 lux of blue light
once for 30 min/day and to 100 lux of blue light once and
twice for 15 min/day could enhance RUM, MOM, MS, and
AK production and reduce RUN and MON compared with
non-exposure. Exposure to 100 lux twice for 30 min/day
and to 200 lux once for 45 min/day decreased the RUM,
MOM, MS, and AK yields and increased the RUN and MON.
Meanwhile, the expression levels of pigment biosynthetic
genes were analyzed by real-time quantitative PCR. Results
indicated that gene MpPKS5, mppR1, mppA, mppB, mmpC,
mppD , MpFasA, MpFasB, and mppF were positively correlated
with the yields of RUN and MON, whereas mppE and
mppR2 were associated with RUM, MOM, MS, and AK
production. |