Title |
MINIREVIEW] Hydroxylation of methane through component interactions in soluble methane monooxygenases |
Author |
Seung Jae Lee |
Address |
Department of Chemistry and Research Institute of Physics and Chemistry, Chonbuk National University, Jeonju 54896, Republic of Korea |
Bibliography |
Journal of Microbiology, 54(4),277-282, 2016,
|
DOI |
10.1007/s12275-016-5642-6
|
Key Words |
methane monooxygenase, oxydoreductase, diiron
active site, reductase, hydroxylation, methane |
Abstract |
Methane hydroxylation through methane monooxygenases
(MMOs) is a key aspect due to their control of the carbon cycle
in the ecology system and recent applications of methane gas
in the field of bioenergy and bioremediation. Methanotropic
bacteria perform a specific microbial conversion from methane,
one of the most stable carbon compounds, to methanol through
elaborate mechanisms. MMOs express particulate methane
monooxygenase (pMMO) in most strains and soluble methane
monooxygenase (sMMO) under copper-limited conditions.
The mechanisms of MMO have been widely studied from
sMMO belonging to the bacterial multicomponent monooxygenase
(BMM) superfamily. This enzyme has diiron active
sites where different types of hydrocarbons are oxidized through
orchestrated hydroxylase, regulatory and reductase components
for precise control of hydrocarbons, oxygen, protons,
and electrons. Recent advances in biophysical studies, including
structural and enzymatic achievements for sMMO, have
explained component interactions, substrate pathways, and
intermediates of sMMO. In this account, oxidation of methane
in sMMO is discussed with recent progress that is critical
for understanding the microbial applications of C-H activation
in one-carbon substrates. |