| Title | Dankookia rubra gen. nov., sp. nov., an alphaproteobacterium isolated from sediment of a shallow stream |
|---|---|
| Author | Wan-Hoe Kim, Do-hak Kim, Keunsoo Kang, and Tae-Young Ahn* |
| Address | Department of Microbiology, College of Natural Sciences, Dankook University, Cheonan 31116, Republic of Korea |
| Bibliography | Journal of Microbiology, 54(6),420-425, 2016, |
| DOI | 10.1007/s12275-016-6054-3 |
| Key Words | Dankookia rubra, alphaproteobacterium |
| Abstract | WS-10T–a Gram-negative, non-motile, and aerobic bacterial strain–was isolated from the sediment of a shallow stream in Korea. The optimum ranges of temperature and pH for growth were 20–40°C (optimum 28°C) and pH 6.0–8.0 (optimum pH 7.0), respectively. The DNA G+C content of strain WS-10T was 72.7 mol%. The major fatty acids (>5%) were summed feature 8 (C18:1 ω7c), summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:0, and C18:1 2-OH. The major polar lipids consisted of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, and unidentified aminolipids. Q-10 was the predominant respiratory quinone. The highest similarities in the 16S rRNA gene sequence were shown with Paracraurococcus ruber (95.3%), Belnapia soli (95.3%), B. moabensis (95.1%), and B. rosea (95.0%). A phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that strain WS-10T formed a distinct line within a clade containing the genera Paracraurococcus, Craurococcus, and Belnapia in the family Acetobacteraceae. On the basis of polyphasic evidence, strain WS-10T represents a novel species of a new genus in the family Acetobacteraceae, for which the name Dankookia rubra gen. nov., sp. nov. is proposed. The type strain of the type species is WS-10T (= KACC 18533T = JCM 30602T). |