Title |
Potential for colonization of O111:H25 atypical enteropathogenic E. coli |
Author |
Marta O. Domingos*, Keyde C.M. Melo, Irys Viana Neves, Cristiane M. Mota, Rita C. Ruiz, Bruna S. Melo, Raphael C. Lima, Denise S.P.Q. Horton, Monamaris M. Borges, and Marcia R. Franzolin |
Address |
Laboratório de Bacteriologia, Instituto Butantan Av. Vital Brazil, 1500, 05503-900, São Paulo, SP, Brazil |
Bibliography |
Journal of Microbiology, 54(11),745-752, 2016,
|
DOI |
10.1007/s12275-016-6015-x
|
Key Words |
atypical enteropathogenic Escherichia coli, aEPEC,
O111:H25 serogroup, virulence factors, biofilm formation,
cat and dogs |
Abstract |
Using clonal phylogenetic methods, it has been demonstrated
that O111:H25 atypical enteropathogenic E. coli (aEPEC)
strains belong to distinct clones, suggesting the possibility
that their ability to interact with different hosts and abiotic
surfaces can vary from one clone to another. Accordingly, the
ability of O111:H25 aEPEC strains derived from human, cat
and dogs to adhere to epithelial cells has been investigated,
along with their ability to interact with macrophages and to
form biofilms on polystyrene, a polymer used to make biomedical
devices. The results demonstrated that all the strains
analyzed were able to adhere to, and to form pedestals on,
epithelial cells, mechanisms used by E. coli to become strongly
attached to the host. The strains also show a Localized-Adherence-
Like (LAL) pattern of adhesion on HEp-2 cells, a
behavior associated with acute infantile diarrhea. In addition,
the O111:H25 aEPEC strains derived either from human
or domestic animals were able to form long filaments,
a phenomenon used by some bacteria to avoid phagocytosis.
O111:H25 aEPEC strains were also encountered inside vacuoles,
a characteristic described for several bacterial strains
as a way of protecting themselves against the environment.
They were also able to induce TNF-α release via two routes,
one dependent on TLR-4 and the other dependent on binding
of Type I fimbriae. These O111:H25 strains were also able
to form biofilms on polystyrene. In summary the results suggest
that, regardless of their source (i.e. linked to human origin
or otherwise), O111:H25 aEPEC strains carry the potential
to cause human disease. |