| Title | Siphonobacter intestinalis sp. nov., a bacterium isolated from the feces of Pseudorhynchus japonicus |
|---|---|
| Author | Shin Ae Lee1, Jeong Myeong Kim1, Jae-Hyung Ahn1, Jae-Ho Joa2, Soo-Jin Kim1, Mee-Kyung Sang1, Jaekyeong Song1, Soon-Wo Kwon1, and Hang-Yeon Weon1* |
| Address | 1Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, Republic of Korea, 2Research Institute of Climate Change and Agriculture, National Institute of Horticultural and Herbal Science, RDA, Jeju 63240, Republic of Korea |
| Bibliography | Journal of Microbiology, 54(11),709-712, 2016, |
| DOI | 10.1007/s12275-016-6451-7 |
| Key Words | Siphonobacter, strain 63MJ-2, novel species, polyphasic taxonomy |
| Abstract | Strain 63MJ-2T was isolated from the feces of broad-winged katydid (Pseudorhynchus japonicus) collected in Korea. The 16S rRNA gene sequence of this strain showed the highest sequence similarity with that of Siphonobacter aquaeclarae P2T (96.1%) and had low similarities (below 86.3%) with those of other members of family ‘Flexibacteraceae’. The strain 63MJ-2T is a strictly aerobic, Gram-stain-negative, non-motile, rod-shaped bacterium. The strain grew at 4–35°C (optimum, 25–30°C), pH of 5.0–9.0 (optimum, 6.0–7.0), and 0–2.0% (optimum, 1.0–2.0) (w/v) NaCl. The DNA G+C content of strain 63MJ-2T was 43.5 mol%. The major fatty acids were C16:1 ω5c (42.5%), iso-C17:0 3-OH (18.7%), and summed feature 3 (iso-C15:0 2-OH and/or C16:1 ω7c, 18.0%). The major menaquinone was MK-7 and polar lipids were phosphatidylethanolamine, six unknown aminolipids, and five unknown lipids. Based on the evidence from our polyphasic taxonomic study, we conclude that strain 63MJ-2T should be classified as a novel species of the genus Siphonobacter, and propose the name Siphonobacter intestinalis sp. nov. The type strain is 63MJ-2T (=KACC 18663T =NBRC 111883T). |