Title |
Siphonobacter intestinalis sp. nov., a bacterium isolated from the feces of Pseudorhynchus japonicus |
Author |
Shin Ae Lee1, Jeong Myeong Kim1, Jae-Hyung Ahn1, Jae-Ho Joa2, Soo-Jin Kim1, Mee-Kyung Sang1, Jaekyeong Song1, Soon-Wo Kwon1, and Hang-Yeon Weon1* |
Address |
1Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, Republic of Korea, 2Research Institute of Climate Change and Agriculture, National Institute of Horticultural and Herbal Science, RDA, Jeju 63240, Republic of Korea |
Bibliography |
Journal of Microbiology, 54(11),709-712, 2016,
|
DOI |
10.1007/s12275-016-6451-7
|
Key Words |
Siphonobacter, strain 63MJ-2, novel species, polyphasic
taxonomy |
Abstract |
Strain 63MJ-2T was isolated from the feces of broad-winged
katydid (Pseudorhynchus japonicus) collected in Korea. The
16S rRNA gene sequence of this strain showed the highest
sequence similarity with that of Siphonobacter aquaeclarae
P2T (96.1%) and had low similarities (below 86.3%) with those
of other members of family ‘Flexibacteraceae’. The strain
63MJ-2T is a strictly aerobic, Gram-stain-negative, non-motile,
rod-shaped bacterium. The strain grew at 4–35°C (optimum,
25–30°C), pH of 5.0–9.0 (optimum, 6.0–7.0), and
0–2.0% (optimum, 1.0–2.0) (w/v) NaCl. The DNA G+C content
of strain 63MJ-2T was 43.5 mol%. The major fatty acids
were C16:1 ω5c (42.5%), iso-C17:0 3-OH (18.7%), and summed
feature 3 (iso-C15:0 2-OH and/or C16:1 ω7c, 18.0%). The major
menaquinone was MK-7 and polar lipids were phosphatidylethanolamine,
six unknown aminolipids, and five unknown
lipids. Based on the evidence from our polyphasic
taxonomic study, we conclude that strain 63MJ-2T should be
classified as a novel species of the genus Siphonobacter, and
propose the name Siphonobacter intestinalis sp. nov. The
type strain is 63MJ-2T (=KACC 18663T =NBRC 111883T). |