Title Deinococcus sedimenti sp. nov. isolated from river sediment
Author Jae-Jin Lee1, Yeon-Hee Lee1, Su-Jin Park1, Sangyong Lim2, Sun-Wook Jeong2, Seung-Yeol Lee1, Sangkyu Park1, Hyo-Won Choi3, Myung Kyum Kim4, and Hee-Young Jung1,5*
Address 1School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea, 2Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, 3National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, Republic of Korea, 4Department of Bio and Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul 01797, Republic of Korea, 5Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea
Bibliography Journal of Microbiology, 54(12),802-808, 2016,
DOI 10.1007/s12275-016-6361-8
Key Words Deinococcus, polyphasic taxonomy, Gamma radiation-resistance
Abstract A novel Gram-positive, oval-shaped, non-motile bacterium designated strain 16F1LT was isolated from sediment collected from the Han River in Seoul, Republic of Korea. Based on the 16S rRNA gene sequence (1,448 bp), this strain was identified as a member of the genus Deinococcus that belongs to the class Deinococci. Similarities in the 16S rRNA gene sequence were shown with Deinococcus daejeonensis MJ27T (99.0%), D. grandis DSM 3963T (98.1%), D. radiotolerans C1T (97.5%), and D. caeni Ho-08T (97.2%). Strain 16F1LT was classified as a different genomic species from closely related Deinococcus members, based on less than 70% DNA-DNA relatedness. Genomic DNA G+C content of strain 16F1LT was 67.2 mol%. Strain 16F1LT was found to grow at temperatures of 10–37°C (optimum 25°C) and pH 7–8 (optimum pH 7) on R2A medium, and was catalase-positive and oxidase-negative. Strain 16F1LT showed resistance to gamma radiation (D10 > 2 kGy). In addition, this strain had the following chemotaxonomic characteristics: the major fatty acids were C15:1 ω6c and C16:1 ω7c; the polar lipid profile contained phosphoglycolipids, unknown aminophospholipids, an unknown aminoglycolipid, unknown aminolipids, an unknown glycolipid, an unknown phospholipid, and an unknown polar lipid; the major quinone was MK-8. Phylogenetic, genotypic, phenotypic, and chemotaxonomic characteristics indicated that strain 16F1LT represents a novel species within the genus Deinococcus, for which the name Deinococcus sedimenti sp. nov. is proposed. The type strain is 16F1LT (=KCTC 33796T =JCM 31405T).