Title |
Deinococcus sedimenti sp. nov. isolated from river sediment |
Author |
Jae-Jin Lee1, Yeon-Hee Lee1, Su-Jin Park1, Sangyong Lim2, Sun-Wook Jeong2, Seung-Yeol Lee1, Sangkyu Park1, Hyo-Won Choi3, Myung Kyum Kim4, and Hee-Young Jung1,5* |
Address |
1School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea, 2Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, 3National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, Republic of Korea, 4Department of Bio and Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul 01797, Republic of Korea, 5Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea |
Bibliography |
Journal of Microbiology, 54(12),802-808, 2016,
|
DOI |
10.1007/s12275-016-6361-8
|
Key Words |
Deinococcus, polyphasic taxonomy, Gamma radiation-resistance |
Abstract |
A novel Gram-positive, oval-shaped, non-motile bacterium
designated strain 16F1LT was isolated from sediment collected
from the Han River in Seoul, Republic of Korea. Based on the
16S rRNA gene sequence (1,448 bp), this strain was identified
as a member of the genus Deinococcus that belongs to the class
Deinococci. Similarities in the 16S rRNA gene sequence were
shown with Deinococcus daejeonensis MJ27T (99.0%), D. grandis
DSM 3963T (98.1%), D. radiotolerans C1T (97.5%), and D.
caeni Ho-08T (97.2%). Strain 16F1LT was classified as a different
genomic species from closely related Deinococcus members,
based on less than 70% DNA-DNA relatedness. Genomic
DNA G+C content of strain 16F1LT was 67.2 mol%. Strain
16F1LT was found to grow at temperatures of 10–37°C (optimum
25°C) and pH 7–8 (optimum pH 7) on R2A medium,
and was catalase-positive and oxidase-negative. Strain 16F1LT
showed resistance to gamma radiation (D10 > 2 kGy). In addition,
this strain had the following chemotaxonomic characteristics:
the major fatty acids were C15:1 ω6c and C16:1 ω7c; the
polar lipid profile contained phosphoglycolipids, unknown
aminophospholipids, an unknown aminoglycolipid, unknown
aminolipids, an unknown glycolipid, an unknown phospholipid,
and an unknown polar lipid; the major quinone was
MK-8. Phylogenetic, genotypic, phenotypic, and chemotaxonomic
characteristics indicated that strain 16F1LT represents
a novel species within the genus Deinococcus, for which the
name Deinococcus sedimenti sp. nov. is proposed. The type
strain is 16F1LT (=KCTC 33796T =JCM 31405T). |