Title A lactic acid bacterium isolated from kimchi ameliorates intestinal inflammation in DSS-induced colitis
Author Jin-Soo Park1, Inseong Joe2, Paul Dong Rhee3, Choon-Soo Jeong1*, and Gajin Jeong3*
Address 1Department of Biological Science, University of Ulsan, Ulsan 44610, Republic of Korea, 2Franklin W. Olin College of Engineering, MA, USA, 3School of Biological Sciences, College of Natural Science, Seoul National University, Seoul 08826, Republic of Korea
Bibliography Journal of Microbiology, 55(4),304-310, 2017,
DOI 10.1007/s12275-017-6447-y
Key Words lactic acid bacteria, kimchi, inflammatory bowel disease, dextran sulfate sodium
Abstract Some species of lactic acid bacteria have been shown to be beneficial in inflammatory bowel disease (IBD). In the pre-sent study, a strain of lactic acid bacterium (Lactobacillus paracasei LS2) was isolated from the Korean food, kimchi, and was shown to inhibit the development of experimental colitis induced by dextran sulfate sodium (DSS). To inves-tigate the role of LS2 in IBD, mice were fed DSS in drinking water for seven days along with LS2 bacteria which were administered intragastrically to some of the mice, while phos-phate-buffered saline (PBS) was administered to others (the controls). The administration of LS2 reduced body weight loss and increased survival, and disease activity indexes (DAI) and histological scores indicated that the severity of colitis was significantly reduced. The production of inflammatory cy-tokines and myeloperoxidase (MPO) activity also decreased. Flow cytometry analysis showed that the number of Th1 (IFN-γ) population cells was significantly reduced in the LS2- administered mice compared with the controls. The admini-stration of LS2 induced the increase of CD4+FOXP3+ Treg cells, which are responsible for IL-10. Numbers of macro-phages (CD11b+ F4/80+), and neutrophils (CD11b+ Gr-1+) among lamina propria lymphocytes (LPL) were also reduced. These results indicate that LS2 has an anti-inflammatory effect and ameliorates DSS-induced colitis.