Title Antibacterial compound produced by Pseudomonas aeruginosa strain UICC B-40, an endophytic bacterium isolated from Neesia altissima
Author Rina Hidayati Pratiwi1,2*, Iman Hidayat3, Muhammad Hanafi4, and Wibowo Mangunwardoyo2
Address 1Department of Biological Education, Faculty of Technics, Mathematics and Natural Sciences, University of Indraprasta PGRI, South Jakarta 12530, Indonesia, 2Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok 16424, Indonesia, 3Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Cibinong 16911, Indonesia, 4Research Center for Chemistry, Indonesian Institute of Sciences (LIPI), PUSPIPTEK, Serpong 15314, Indonesia
Bibliography Journal of Microbiology, 55(4),289-295, 2017,
DOI 10.1007/s12275-017-6311-0
Key Words antibacterial, bioactive, endophytic bacteria, Neesia altissima, Pseudomonas aeruginosa strain UICC B-40
Abstract This study’s aim was to determine the identity of antibacte-rial compounds produced by Pseudomonas aeruginosa strain UICC B-40 and describe the antibacterial compounds’ me-chanisms of action for damaging pathogenic bacteria cells. Isolation and identification of the compounds were carried out using thin layer chromatography (TLC), nuclear mag-netic resonance (NMR) spectroscopy and liquid chromato-graphy mass spectrometry (LC-MS) analyses. Antibacterial activity was assayed via minimum inhibitory concentration (MIC) and the antibacterial compound mechanism was ob-served morphologically through scanning electron micros-copy (SEM). This study successfully identified the (2E,5E)- phenyltetradeca-2,5-dienoate antibacterial compound (mole-cular weight 300 g/mol), composed of a phenolic ester, fatty acid and long chain of aliphatic group structures. MIC values for this compound were determined at 62.5 μg/ml against Staphylococcus aureus strain ATCC 25923. The mechanism of the compound involved breaking down the bacterial cell walls through the lysis process. The (2E,5E)-phenyltetradeca- 2,5-dienoate compound exhibited inhibitory activity on the growth of Gram-positive bacteria.