| Title | Alteration in the ultrastructural morphology of mycelial hyphae and the dynamics of transcriptional activity of lytic enzyme genes during basidiomycete morphogenesis |
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| Author | Elena Vetchinkina1*, Maria Kupryashina1, Vladimir Gorshkov2,3, Marina Ageeva2, Yuri Gogolev3,4, and Valentina Nikitina1 |
| Address | 1Laboratory of Microbiology, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences IBPPM RAS, 13 Prospekt Entuziastov, Saratov 410049, Russian Federation, 2Laboratory of Molecular Biology, Kazan Institute of Biochemistry and Biophysics, Kazan Science Center, Russian Academy of Sciences, 2/31 Lobachevsky street, Kazan 420111, Russian Federation, 3Kazan Federal University, 18 Kremlyovskaya street, Kazan 420008, Russian Federation, 42Laboratory of Molecular Biology, Kazan Institute of Biochemistry and Biophysics, Kazan Science Center, Russian Academy of Sciences, 2/31 Lobachevsky street, Kazan 420111, Russian Federation |
| Bibliography | Journal of Microbiology, 55(4),280-288, 2017, |
| DOI | 10.1007/s12275-017-6320-z |
| Key Words | basidiomycete morphogenesis, gene expression, lytic enzymes, phenol oxidases, cell wall ultrastructure |
| Abstract | The morphogenesis of macromycetes is a complex multilevel process resulting in a set of molecular-genetic, physiological- biochemical, and morphological-ultrastructural changes in the cells. When the xylotrophic basidiomycetes Lentinus edodes, Grifola frondosa, and Ganoderma lucidum were grown on wood waste as the substrate, the ultrastructural morphology of the mycelial hyphal cell walls differed considerably between mycelium and morphostructures. As the macromycetes passed from vegetative to generative development, the expression of the tyr1, tyr2, chi1, chi2, exg1, exg2, and exg3 genes was acti-vated. These genes encode enzymes such as tyrosinase, chi-tinase, and glucanase, which play essential roles in cell wall growth and morphogenesis. |