Title Hymenobacter daeguensis sp. nov. isolated from river water

Leonid N. Ten1, Yeon-Hee Lee1, Jae-Jin Lee1, Su-Jin Park1, Seung-Yeol Lee1, Sangkyu Park1, Dae Sung Lee2, In-Kyu Kang3, and Hee-Young Jung1,4*

Address 1School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea, 2Department of Environmental Engineering, Kyungpook National University, Daegu 41566, Republic of Korea, 3Department of Horticultural Science, Kyungpook National University, Daegu 41566, Republic of Korea, 4Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea
Bibliography Journal of Microbiology, 55(4),253-259, 2017,
DOI 10.1007/s12275-017-6524-2
Key Words Hymenobacter, Bacteroidetes, polyphasic taxo-nomy
Abstract A Gram-stain-negative, non-motile, non-spore-forming, rod- shaped, aerobic bacterial strain, designated 16F3Y-2T, was isolated from the Han River, South Korea, and was charac-terized taxonomically using a polyphasic approach. Compa-rative 16S rRNA gene sequence analysis showed that strain 16F3Y-2T belonged to the family Cytophagaceae in the phy-lum Bacteroidetes and was most closely related to ‘Hymeno-bacter terrae’ DG7A (98.01%), H. soli PB17T (97.26%), H. glaciei VUG-A130T (96.78%), H. antarcticus VUG-A42aaT (96.72%), H. ruber PB156T (96.61%), and H. saemangeumensis GSR0100T (95.77%). The G+C content of the genomic DNA of strain 16F3Y-2T was 62.9 mol%. The isolate contained MK-7 as the predominant respiratory quinone, and summed fea-ture 3 (C16:1 ω7c/C16:1 ω6c; 35.5%), C15:0 iso (16.9%), C16:1 ω5c (10.9%), and C15:0 anteiso (9.9%) as major fatty acids. The ma-jor polar lipid was phosphatidylethanolamine. Phenotypic and chemotaxonomic data supported the affiliation of strain 16F3Y-2T with the genus Hymenobacter. However, strain 16F3Y-2T exhibited relatively low levels of DNA-DNA related-ness with 'H. terrae' KCTC 32554 (44.1%) and H. soli KCTC 12607T (24.3%), clearly indicating that the isolate constitutes a new genospecies. Strain 16F3Y-2T could be differentiated from its phylogenetic neighbors on the basis of several phe-notypic, genotypic, and chemotaxonomic features. Therefore, strain 16F3Y-2T represents a novel species in the genus Hy-menobacter, for which the name Hymenobacter daeguensis sp. nov. is proposed. The type strain is 16F3Y-2T (=KCTC 52537T =JCM 31654T).