Title Spirosoma luteolum sp. nov. isolated from water
Author Jae-Jin Lee1, Su-Jin Park1, Yeon-Hee Lee1, Seung-Yeol Lee1, Sangkyu Park1, Young-Je Cho2, Myung Kyum Kim3, Leonid N. Ten1, and Hee-Young Jung4*
Address 1School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea, 2School of Food Science and Biotechnology/Food and Bio-Industry Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea, 3Department of Bio and Environmental Technology, Seoul Women’s University, Seoul 01797, Republic of Korea, 4Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea
Bibliography Journal of Microbiology, 55(4),247-252, 2017,
DOI 10.1007/s12275-017-6455-y
Key Words Spirosoma, polyphasic taxonomy, water
Abstract A novel Gram-negative and rod-shaped bacterial strain, de-signated as 16F6ET, was isolated from a water sample. Cells were yellowish in color and catalase- and oxidase-positive. The strain grew at 10–37°C (optimum at 25°C) but not at 4 and 42°C, and pH 5–7 (optimum at pH 7). It showed mod-erate resistance to gamma-ray irradiation. Comparative phy-logenetic analysis showed that strain 16F6ET belonged to the family Cytophagaceae of the class Cytophagia. Furthermore, this isolate showed relatively low 16S rRNA gene sequence similarities (90.7–93.1%) to the members of the genus Spiro-soma. The major fatty acids were summed feature 3 (C16:1 ω7c/C16:1 ω6c), C16:1 ω5c, C16:0 N alcohol, and C16:0. The polar lipid profile indicated presence of phosphatidylethanolamine, unknown aminophospholipids, an unknown amino lipid, unknown phospholipids, and unknown polar lipids. The pre-dominant isoprenoid quinone was MK-7. The genomic DNA G+C content of strain 16F6ET was 56.5 mol%. Phenotypic, phylogenetic, and chemotaxonomic properties indicated that isolate 16F6ET represents a novel species within the genus Spirosoma, for which the name Spirosoma luteolum sp. nov. is proposed. The type strain is 16F6ET (=KCTC 52199T =JCM 31411T).