Title |
Minimal amino acids in the I/LWEQ domain required for anterior/posterior localization in Dictyostelium |
Author |
Hyeseon Kim, Dong-Yeop Shin, and Taeck Joong Jeon* |
Address |
Department of Biology & BK21-Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University, Gwangju 61452, Republic of Korea |
Bibliography |
Journal of Microbiology, 55(5),366-372, 2017,
|
DOI |
10.1007/s12275-017-6550-0
|
Key Words |
cell polarity, cell migration, Dictyostelium, I/LWEQ domain, RapGAP3 |
Abstract |
Establishment of cell polarity is mediated by a series of signal-ing molecules that are asymmetrically activated or localized in the cell upon extracellular stimulation. To understand the mechanism that mediates anterior/posterior asymmetric localization of RapGAP3 during migration, we determined the minimally required amino acids in the I/LWEQ domain that cause posterior localization and found that the minimal region of the F-actin binding domain for posterior localiza-tion could, with some additional deletion at the C-terminal, localize to the anterior. Analysis of the localization and trans-location kinetics to the cell cortex of the truncated proteins suggests that the required regions for anterior/posterior lo-calization might have a preferential binding affinity to pre- existing F-actins at the rear and lateral sides of the cell or newly formed F-actins at the front of the cell, leading to dis-tinct differential sites of the cell. |