| Title | Coptidis Rhizoma extract inhibits replication of respiratory syncytial virus in vitro and in vivo by inducing antiviral state |
|---|---|
| Author | Byeong-Hoon Lee1, Kiramage Chathuranga1, Md Bashir Uddin1,2, Prasanna Weeratunga1, Myun Soo Kim3, Won-Kyung Cho4, Hong Ik Kim3, Jin Yeul Ma4, and Jong-Soo Lee1* |
| Address | 1College of Veterinary Medicine, Chungnam National University, 2Faculty of Veterinary and Animal Science, Sylhet Agricultural, 3Vitabio Corporation, Daejeon 34540, Republic of Korea, 4Korean Medicine (KM) Application Center, Korea Institute of Oriental |
| Bibliography | Journal of Microbiology, 55(6),488-498, 2017, |
| DOI | 10.1007/s12275-017-7088-x |
| Key Words | coptidis rhizoma (CR), palmatine, respiratory syncytial virus (RSV), antiviral effect |
| Abstract | Coptidis Rhizoma is derived from the dried rhizome of Ranun-culaceous plants and is a commonly used traditional Chinese medicine. Although Coptidis Rhizoma is commonly used for its many therapeutic effects, antiviral activity against respi-ratory syncytial virus (RSV) has not been reported in detail. In this study, we evaluated the antiviral activities of Coptidis Rhizoma extract (CRE) against RSV in human respiratory tract cell line (HEp2) and BALB/c mice. An effective dose of CRE significantly reduces the replication of RSV in HEp2 cells and reduces the RSV-induced cell death. This antiviral activity against RSV was through the induction of type I inter-feron-related signaling and the antiviral state in HEp2 cells. More importantly, oral administration of CRE exhibited prophylactic effects in BALB/c mice against RSV. In HPLC analysis, we found the presence of several compounds in the aqueous fraction and among them; we confirmed that pal-matine was related to the antiviral properties and immune- modulation effect. Taken together, an extract of Coptidis Rhi-zoma and its components play roles as immunomodulators and could be a potential source as promising natural antivirals that can confer protection to RSV. These outcomes should encourage further allied studies in other natural products. |