Title |
Identification of cyst wall proteins of the hypotrich ciliate Euplotes encysticus using a proteomics approach |
Author |
Bangzheng Wang1, Tao Niu1, Muhammad Zeeshan Bhatti2, Fenfen Chen1, Lin Wu1, and Jiwu Chen1* |
Address |
1School of Life Sciences, East China Normal University, Shanghai 200241, P. R. China, 2Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai 200241, P. R. China |
Bibliography |
Journal of Microbiology, 55(7),545–553, 2017,
|
DOI |
10.1007/s12275-017-6422-7
|
Key Words |
Euplotes encysticus, encystment, cyst wall proteins,
mass spectrum identification, fluorescence taxtoid |
Abstract |
Euplotes encysticus is a species of Hypotrich ciliates, which
form cyst wall by secreting the special substances on encounter
of adverse environment. It has critical significance to study
the component and mechanism underlying resting cyst, during
resisting unfavorable conditions in dormancy induction.
The present study was aimed to investigate the effects of cyst
wall proteins of Euplotes encysticus by using biochemical
methods. Therefore, protein extracts were separated by SDSPAGE,
identified and analyzed by MALDI-TOF MS and Bioinformatics
tools. We detected 42 cyst wall proteins, 26 were
functional proteins and 16 proteins consist of unknown function;
which is consistent with cyst wall specificity. These results
partially revealed the components of resting cyst wall
formed after the cells differentiation of Euplotes encysticus.
In addition, our data suggested that the function of cyst wall
proteins are more likely involved in the mechanical protection,
signal transduction, material transport, protein degradation
and energy metabolism to survival, with potentially
importance implications in the molecular mechanism of eukaryocyte
dormancy under stress condition. |