Title Mucilaginibacter hankyongensis sp. nov., isolated from soil of ginseng field Baekdu Mountain
Author Qingmei Liu1,2, Muhammad Zubair Siddiqi1,2, Mi-Sun Kim3, Sang Yong Kim4, and Wan-Taek Im1,2*
Address 1Department of Biotechnology, Hankyong National University, Anseong 17579, Republic of Korea, 2Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyong National University, Anseong 17579, Republic of Korea, 3Clean Fuel Research Center, Korea Institute of Energy Research, Daejeon 34129, Republic of Korea, 4Department of Food Science & Bio Technology, Shinansan University, Ansan 15435, Republic of Korea
Bibliography Journal of Microbiology, 55(7),525–530, 2017,
DOI 10.1007/s12275-017-7180-2
Key Words 16S rRNA gene, polyphasic taxonomy, Mucilaginibacter hankyongensis, ginsenoside
Abstract A Gram-negative, non-motile, aerobic, and rod-shaped bacterial strain designated as BR5-28T was isolated from the soil of a ginseng field at Baekdu Mountain Korea, and its taxonomic position was investigated using a polyphasic approach. Strain BR5-28T grew at 10–42°C (optimum temperature, 30°C) and pH 5.5–8.5 (optimum pH, 7.0) on R2A agar medium without additional NaCl supplementation. Strain BR5- 28T exhibited β-glucosidase activity, which was responsible for its ability to transform the ginsenosides Rb1 and Rd (the two dominant active components of ginseng) to compound-K. Based on 16S rRNA gene phylogeny, the novel strain showed a new branch within the genus Mucilaginibacter of the family Sphingobacteriaceae, and formed clusters with Mucilaginibacter frigoritolerans FT22T (95.8%) and Mucilaginibacter gotjawali SA3-7T (95.7%). The G+C content of the genomic DNA was 45.1%. The predominant respiratory quinone was MK-7 and the major fatty acids were summed feature 3 (comprising C16:1 ω6c and/or C16:1 ω7c), iso-C15:0 and anteiso-C15:0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Strain BR5- 28T was differentiated genotypically and phenotypically from the recognized species of the genus Mucilaginibacter. The isolate therefore represents a novel species, for which the name Mucilaginibacter hankyongensis sp. nov. is proposed, with the type strain BR5-28T (=KCTC 22274T =DSM 21151T).