Title |
Ensifer collicola sp. nov., a bacterium isolated from soil in South Korea |
Author |
Jun Hyeong Jang, Dongwook Lee, Seho Cha, and Taegun Seo* |
Address |
Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea |
Bibliography |
Journal of Microbiology, 55(7),520–524, 2017,
|
DOI |
10.1007/s12275-017-6608-z
|
Key Words |
Ensifer, taxonomy, Rhizobiaceae, Ensifer collicola
sp. nov. |
Abstract |
Strain Mol12T, which presented in the form of Gram-negative,
motile, non-spore forming rod-shaped, was isolated from
soil in South Korea and characterized to determine its taxonomic
position. The strain grew at 20–30°C (optimum 30°C)
and pH 7.0–10.0 (optimum pH 8.0) with 1% (w/v) NaCl.
Phylogenetic analysis based on 16S rRNA gene sequences
showed that the strain Mol12T was most closely related to
Ensifer terangae LMG 7834T (96.78%), Rhizobium daejeonense
KCTC 12121T (96.43%), Ensifer adhaerens Casida AT
(96.28%). Chemotaxonomic data showed that the predominant
fatty acids were Summed Feature 8 (C18:1 ω7c and/or
C18:1 ω6c; 53.02%) and C18:1 ω7c 11-methyl (24.01%). Its complex
polar lipid contained major amounts of diphosphatidylglycerol
(DPG), phosphatidylethanolamine (PE) and Q-10
as the predominant ubiquinone. The DNA G+C content of
strain Mol12T was determined to be 60.9 mol%. Based on
the phylogenetic, chemotaxonomic, and phenotypic data,
strain Mol12T (=KCTC 42816T =JCM 31049T) ought to be
classified as a type strain of a novel species, for which the
name Ensifer collicola sp. nov. is proposed. |