Title |
Limnobacter humi sp. nov., a thiosulfate-oxidizing, heterotrophic bacterium isolated from humus soil, and emended description of the genus Limnobacter Spring et al. 2001 |
Author |
Tuan Manh Nguyen1,2 and Jaisoo Kim1* |
Address |
1Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon 16227, Republic of Korea, 2Thai Nguyen University of Agriculture and Forestry, Quyet Thang Commune, Thai Nguyen, Vietnam |
Bibliography |
Journal of Microbiology, 55(7),508–513, 2017,
|
DOI |
10.1007/s12275-017-6645-7
|
Key Words |
Limnobacter humi sp. nov., taxonomy, chemolithoheterotrophic,
thiosulfate oxidizing |
Abstract |
Three Gram-negative, strictly aerobic, chemolithoheterotrophic
bacterial strains, designated UCM-30, UCM-33, and
UCM-39T, were isolated in South Korea. Based on their 16S
rRNA gene sequences, the three isolated strains were found
to be similar to Limnobacter thiooxidans CS-K2T (97.41–
97.68%), Limnobacter litoralis KP1-19T (95.55–95.76%), and
various genera belonging to the class Betaproteobacteria
(90.34–93.34%). DNA-DNA hybridization showed 79.3–83.9%
similarity between the genomic DNA of UCM-39T, UCM-30,
and UCM-33, while the sequence similarity between UCM-
39T and L. thiooxidans KACC 13837T or L. litoralis LMG
24869T was 23.7% and 18.6%, respectively. The DNA G+C
content of UCM 39T was 59.7 mol%, the major ubiquinone
was Q-8, and the optimal oxidation rate was observed at 10
mM thiosulfate. The major fatty acids (≥ 10%) were summed
features 3 (C16:1ω7c and/or C16:1ω6c) and 8 (C18:1ω7c and/or
C18:1ω6c), and C16:0. The major polar lipids (diphosphatidylglycerol,
phosphatidylethanolamine, and phosphatidylglycerol)
were found in all members of genus Limnobacter. Based
on phenotypic, physiological, and phylogenetic analyses, the
UCM-39T strain was found to be significantly distinct to represent
a novel species affiliated to the genus Limnobacter. We
propose to name it Limnobacter humi sp. nov. with the type
strain UCM-39T (=KACC 18574T =NBRC 111650T). |