| Abstract |
Vibrio parahaemolyticus is a halophilic bacterium endemic
to coastal areas, and its pathogenicity has caused widespread
seafood poisoning. In our previous research, the protein expression
of V. parahaemolyticus in Fe3+ medium was determined
using isobaric tags for relative and absolute quantitation
(iTRAQ). Here, nuclear magnetic resonance (NMR) was
used to detect changes in the V. parahaemolyticus metabolome.
NMR spectra were obtained using methanol-water extracts
of intracellular metabolites from V. parahaemolyticus under
various culture conditions, and 62 metabolites were identified,
including serine, arginine, alanine, ornithine, tryptophan,
glutamine, malate, NAD+, NADP+, oxypurinol, xanthosine,
dCTP, uracil, thymine, hypoxanthine, and betaine. Among
these, 21 metabolites were up-regulated after the stimulation
of the cells by ferric iron, and 9 metabolites were down-regulated.
These metabolites are involved in amino acid and protein
synthesis, energy metabolism, DNA and RNA synthesis
and osmolality. Based on these results, we conclude that Fe3+
influences the metabolite profiles of V. parahaemolyticus. |
