| Title | Imipenem-resistant Gram-negative bacterial isolates carried by persons upon medical examination in Korea |
|---|---|
| Author | So Yeon Kim1, Sang Yop Shin2, Ji-Young Rhee3, and Kwan Soo Ko1* |
| Address | 1Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of Korea, 2Korea Medical Institute, Seoul 03173, Republic of Korea, 3Division of Infectious Diseases, Department of Medicine, Dankook University, Cheonan 31116, Republic of Korea |
| Bibliography | Journal of Microbiology, 55(8),612–618, 2017, |
| DOI | 10.1007/s12275-017-6555-8 |
| Key Words | Imipenem resistance, fecal carriage, Proteus mirabilis, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Enterobacter cloacae |
| Abstract | Carbapenem-resistant Gram-negative bacteria (CR-GNB) have emerged and disseminated worldwide, become a great concern worldwide including Korea. The prevalence of fecal carriage of imipenem-resistant Gram-negative bacteria (IRGNB) in persons in Korea was investigated. Stool samples were collected from 300 persons upon medical examination. Samples were screened for IR-GNB by using MacConkey agar with 2 μl/ml imipenem. Species were identified by 16S rRNA gene sequence analysis, and antimicrobial susceptibility was determined by the broth microdilution method. In total, 82 IR-GNB bacterial isolates were obtained from 79 (26.3%) out of 300 healthy persons. Multilocus sequence typing analysis showed very high diversity among IR P. aeruginosa, S. maltophilia, and E. cloacae isolates, and pulsedfield gel electrophoresis revealed five main pulsotypes of IR P. mirabilis. As for the presence of metallo-β-lactamases (MBLs), only one IMP-25-producing S. marcescens isolate was identified. Although only one carbapenemase-producing isolate was identified, the high colonization rates with IRGNB isolates in this study is notable because carriers may be a reservoir for the dissemination of resistant pathogens within the community as well as in health care institutions. |