Title |
Pseudaminobacter granuli sp. nov., isolated from granules used in a wastewater treatment plant |
Author |
Young Ki Hahn1, Minseok S. Kim2*, and Wan-Taek Im3,4* |
Address |
1Samsung Electronics, Seoul 06620, Republic of Korea, 2Department of New Biology, Daegu Gyeongbuk Institute of Science & Technology (DGIST), Daegu 42988, Republic of Korea, 3Department of Biotechnology, Hankyong National University, Kyonggi-do 17579, Republic of Korea, 4Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyong National University, Kyonggi-do 17579, Republic of Korea |
Bibliography |
Journal of Microbiology, 55(8),607–611, 2017,
|
DOI |
10.1007/s12275-017-7257-y
|
Key Words |
16S rRNA gene, polyphasic taxonomy, Pseudaminobacter
granuli, granules |
Abstract |
A Gram negative, aerobic, non-motile and rod-shaped bacterial
strain designated as Gr-2T was isolated from granules
used in a wastewater treatment plant in Korea, and its taxonomic
position was investigated using a polyphasic approach.
Strain Gr-2T grew at 18–37°C (optimum temperature, 30°C)
and a pH of 6.0–8.0 (optimum pH, 7.0) on R2A agar medium.
Based on 16S rRNA gene phylogeny, the novel strain showed
a new branch within the genus Pseudaminobacter of the family
Phyllobacteriaceae, and formed clusters with Pseudaminobacter
defluvii THI 051T (98.9%) and Pseudaminobacter
salicylatoxidans BN12T (98.7%). The G+C content of the genomic
DNA was 63.6%. The predominant respiratory quinone
was ubiquinone-10 (Q-10) and the major fatty acids were
cyclo-C19:0 ω8c, C18:1 ω7c, and iso-C17:0. The overall polar lipid
patterns of Gr-2T were similar to those determined for the
other Pseudaminobacter species. DNA-DNA relatedness values
between strain Gr-2T and its closest phylogenetically neighbors
were below 18%. Strain Gr-2T could be differentiated genotypically
and phenotypically from the recognized species of the
genus Pseudaminobacter. The isolate therefore represents a
novel species, for which the name Pseudaminobacter granuli
sp. nov. is proposed with the type strain Gr-2T (=KACC 18877T
=LMG 29567T). |