Title |
Bile salt hydrolase-mediated inhibitory effect of Bacteroides ovatus on growth of Clostridium difficile |
Author |
Soobin Yoon1, Junsun Yu1, Andrea McDowell1, Sung Ho Kim2, Hyun Ju You3, and GwangPyo Ko1,2,3 |
Address |
1Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul 08826, Republic of Korea, 2KoBioLabs, Inc., Seoul 08826, Republic of Korea, 3Center for Human and Environmental Microbiome, Institute of Health and Environment, Seoul National University, Seoul 08826, Republic of Korea |
Bibliography |
Journal of Microbiology, 55(11),892–899, 2017,
|
DOI |
10.1007/s12275-017-7340-4
|
Key Words |
Clostridium difficile, bile salt hydrolase, Bacteroides
ovatus |
Abstract |
Clostridium difficile infection (CDI) is one of the most common
nosocomial infections. Dysbiosis of the gut microbiota
due to consumption of antibiotics is a major contributor to
CDI. Recently, fecal microbiota transplantation (FMT) has
been applied to treat CDI. However, FMT has important limitations
including uncontrolled exposure to pathogens and
standardization issues. Therefore, it is necessary to evaluate
alternative treatment methods, such as bacteriotherapy, as
well as the mechanism through which beneficial bacteria inhibit
the growth of C. difficile. Here, we report bile acid-mediated
inhibition of C. difficile by Bacteroides strains which
can produce bile salt hydrolase (BSH). Bacteroides strains
are not commonly used to treat CDI; however, as they comprise
a large proportion of the intestinal microbiota, they can
contribute to bile acid-mediated inhibition of C. difficile. The
inhibitory effect on C. difficile growth increased with increasing
bile acid concentration in the presence of Bacteroides
ovatus SNUG 40239. Furthermore, this inhibitory effect on
C. difficile growth was significantly attenuated when bile acid
availability was reduced by cholestyramine, a bile acid sequestrant.
The findings of this study are important due to
the discovery of a new bacterial strain that in the presence
of available bile acids inhibits growth of C. difficile. These
results will facilitate development of novel bacteriotherapy
strategies to control CDI. |