Title |
Characterization of siderophore produced by Pseudomonas syringae BAF.1 and its inhibitory effects on spore germination and mycelium morphology of Fusarium oxysporum |
Author |
Sumei Yu, Chunying Teng, Jinsong Liang, Tao Song, Liying Dong, Xin Bai, Yu Jin, and Juanjuan Qu* |
Address |
College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, P. R. China |
Bibliography |
Journal of Microbiology, 55(11),877–884, 2017,
|
DOI |
10.1007/s12275-017-7191-z
|
Key Words |
Fusarium oxysporum, Pseudomonas syringae BAF.1,
siderophore, biocontrol |
Abstract |
In this study, an antagonistic bacterium against Fusarium
oxysporum was identified and designated as Pseudomonas
syringae strain BAF.1 on the basis of 16S rDNA sequence
analysis and physiological-biochemical characteristics. It produced
catechol-species siderophore at a molecular weight
of 488.59 Da and a maximum amount of 55.27 μg/ml with
glucose as a carbon source and asparagine as a nitrogen
source at a C/N ratio of 10:1, 30°C and pH 7. The siderophore
exhibited prominent antagonistic activity against Fusarium
oxysporum with a maximum inhibition rate of 95.24% and
had also suppressive effects on other kinds of 11 phytopathogenic
fungi in the absence of FeCl3·6H2O. Spore germination
was completely inhibited by 50 μl of the siderophorecontaining
solution, and the ultrastructures of mycelia and
spores were also considerably suppressed by siderophore
treatment as established by electron microscopy observation.
These results indicate that the siderophore produced by Pseudomonas
syringae BAF.1 could be potentially used for biocontrol
of pathogenic Fusarium oxysporum. |