Title |
De novo transcriptome assembly and characterization of the 10-hydroxycamptothecin-producing Xylaria sp. M71 following salicylic acid treatment |
Author |
Xiaowei Ding1,2, Kaihui Liu2*, Yonggui Zhang2, and Feihu Liu1* |
Address |
1School of Agriculture, Yunnan University, Kunming 650091, P. R. China, 2School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, P. R. China |
Bibliography |
Journal of Microbiology, 55(11),871–876, 2017,
|
DOI |
10.1007/s12275-017-7173-1
|
Key Words |
transcriptomic analysis, Xylaria sp. M71, 10-hydroxycamptothecin |
Abstract |
In the present study, we identified genes that are putatively
involved in the production of fungal 10-hydroxycamptothecin
via transcriptome sequencing and characterization of the
Xylaria sp. M71 treated with salicylic acid (SA). A total of
60,664,200 raw reads were assembled into 26,044 unigenes.
BLAST assigned 8,767 (33.7%) and 10,840 (41.6%) unigenes
to 40 Gene Ontology (GO) annotations and 108 Kyoto Encyclopedia
of Genes and Genomes (KEGG) pathways, respectively.
A total of 3,713 unigenes comprising 1,504 upregulated
and 2,209 downregulated unigenes were found to be differentially
expressed between SA-induced and control fungi.
Based on the camptothecin biosynthesis pathway in plants,
13 functional genes of Xylaria sp. M71 were mapped to the
mevalonate (MVA) pathway, suggesting that the fungal 10-hydroxycamptothecin
is produced via the MVA pathway. In
summary, analysis of the Xylaria sp. M71 transcriptome allowed
the identification of unigenes that are putatively involved
in 10-hydroxycamptothecin biosynthesis in fungi. |