| Title | Spirosoma lituiforme sp. nov., isolated from soil |
|---|---|
| Author | Weilan Li1, Seung-Yeol Lee1, Sangkyu Park1, Byung-Oh Kim2, Leonid N. Ten1, and Hee-Young Jung1,3* |
| Address | 1School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea, 2School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea, 3Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea |
| Bibliography | Journal of Microbiology, 55(11),856–861, 2017, |
| DOI | 10.1007/s12275-017-7255-0 |
| Key Words | Spirosoma, Cytophagaceae, polyphasic taxonomy |
| Abstract | A Gram-staining-negative, non-motile, curved rod-shaped, aerobic bacterium, designated S1-2-4T, was isolated from soil in Jeollabuk-do province, Republic of Korea, and was characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain S1-2-4T was a member of the family Cytophagaceae and most closely related to ‘Spirosoma radiotolerans’ DG5A (97.2%), Spirosoma fluviale MSd3T (96.4%), and Spirosoma linguale DSM 74T (96.3%). The genomic DNA G + C content of strain S1-2-4T was 49.7 mol%. The major fatty acids were summed feature 3 (C16:1 ω7c/C16:1 ω6c), C16:1 ω5c, and C16:0, and the major polar lipid was phosphatidylethanolamine. MK-7 was the predominant respiratory quinone. Phenotypic and chemotaxonomic data supported the affiliation of strain S1-2-4T with the genus Spirosoma. DNA-DNA hybridization between strain S1-2-4T and ‘Spirosoma radiotolerans’ showed relatively low DNA-DNA relatedness (31%). Strain S1-2-4T could be distinguished from its closest phylogenetic neighbors based on its phenotypic, genotypic, and chemotaxonomic features. Therefore, strain S1-2-4T represents a novel member of the genus Spirosoma, for which the name Spirosoma lituiforme sp. nov. is proposed. The type strain is S1- 2-4T (= KCTC 52724T = JCM 32128T). |