| Title | Spirosoma flavus sp. nov., a novel bacterium from soil of Jeju Island |
|---|---|
| Author | Nabil Elderiny1, Seung-Yeol Lee1, Sangkyu Park1, In-Kyu Kang2, Myung Kyum Kim3, Dae Sung Lee4, Leonid N. Ten1, and Hee-Young Jung1,5* |
| Address | 1College of Agricultural and Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea, 2Department of Horticultural Science, Kyungpook National University, Daegu 41566, Republic of Korea, 3Department of Bio and Environmental Technology, Seoul Women’s University, Seoul 01797, Republic of Korea, 4Department of Environmental Engineering, Kyungpook National University, Daegu 41566, Republic of Korea, 5Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea |
| Bibliography | Journal of Microbiology, 55(11),850–855, 2017, |
| DOI | 10.1007/s12275-017-7360-0 |
| Key Words | Spirosoma, Bacteroidetes, soil bacteria |
| Abstract | A novel, Gram-staining negative, yellow pigmented bacterial strain, designated 15J11-2T, was isolated from soil sample on Jeju Island, Republic of Korea. The strain was subjected to a taxonomic study using a polyphasic approach. The strain was able to grow at temperature range from 10°C to 30°C, pH 7–8, and in presence of 0–1% (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain 15J11-2T belongs to the genus Spirosoma and levels of 16S rRNA gene sequence similarity ranged from 91.5% to 89.8%. The genomic DNA G + C content of strain 15J11-2T was 46.0 mol%. The isolate contained phosphatidylethanolamine and an unidentified aminophospholipid as the main polar lipids, menaquinone MK-7 as the predominant respiratory quinone, and summed feature 3 (C16:1 ω6c/C16:1 ω7c; 39.4%), C16:1 ω5c (27.1%), and C16:0 (13.0%) as the major fatty acids, which supported the affiliation of strain 15J11-2T to the genus Spirosoma. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 15J11-2T from recognized Spirosoma species. On the basis of its phenotypic properties, genotypic distinctiveness, chemotaxonomic features, strain 15J11-2T represents a novel species of the genus Spirosoma, for which the name Spirosoma flavus sp. nov. is proposed. The type strain is 15J11-2T (= KCTC 52026T = JCM 31998T). |