Title |
Application of high-salinity stress for enhancing the lipid productivity of Chlorella sorokiniana HS1 in a two-phase process |
Author |
Ramesh Kakarla1, Jung-Woon Choi1, Jin-Ho Yun2, Byung-Hyuk Kim3, Jina Heo1,4, Sujin Lee1,4, Dae-Hyun Cho1, Rishiram Ramanan5, and Hee-Sik Kim1,4* |
Address |
1Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea, 2Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 34141, Republic of Korea, 3Research Institute of Climate Change and Agriculture, National Institute of Horticultural and Herbal Science, RDA, Jeju 63240, Republic of Korea, 4Environmental Biotechnology, University of Science & Technology, Daejeon 34113, Republic of Korea, 5Department of Environmental Science, Central University of Kerala, Padannakkad Campus Kasaragod District, India |
Bibliography |
Journal of Microbiology, 56(1),56–64, 2018,
|
DOI |
10.1007/s12275-018-7488-6
|
Key Words |
Chlorella sorokiniana HS1, high-salinity stress,
lipid induction, microalgae, two-phase process |
Abstract |
Increased lipid accumulation of algal cells as a response to
environmental stress factors attracted much attention of researchers
to incorporate this stress response into industrial
algal cultivation process with the aim of enhancing algal lipid
productivity. This study applies high-salinity stress condition
to a two-phase process in which microalgal cells are initially
grown in freshwater medium until late exponential phase and
subsequently subjected to high-salinity condition that induces
excessive lipid accumulation. Our initial experiment revealed
that the concentrated culture of Chlorella sorokiniana HS1
exhibited the intense fluorescence of Nile red at the NaCl
concentration of 60 g/L along with 1 g/L of supplemental bicarbonate
after 48 h of induction period without significantly
compromising cultural integrity. These conditions were further
verified with the algal culture grown for 7 days in a 1 L
bottle reactor that reached late exponential phase; a 12% increment
in the lipid content of harvested biomass was observed
upon inducing high lipid accumulation in the concentrated
algal culture at the density of 5.0 g DW/L. Although
an increase in the sum of carbohydrate and lipid contents of
harvested biomass indicated that the external carbon source
supplemented during the induction period increased overall
carbon assimilation, a decrease in carbohydrate content suggested
the potential reallocation of cellular carbon that promoted
lipid droplet formation under high-salinity stress. These
results thus emphasize that the two-phase process can be successfully
implemented to enhance algal lipid productivity by incorporating high-salinity stress conditions into the pre-concentrated
sedimentation ponds of industrial algal production
system. |