Title |
MINIREVIEW] Rapid and robust MALDI-TOF MS techniques for microbial identification: a brief overview of their diverse applications |
Author |
Kyoung-Soon Jang1,2* and Young Hwan Kim1,2,3 |
Address |
1Biomedical Omics Group, Korea Basic Science Institute, Cheongju 28119, Republic of Korea, 2Department of Bio-Analytical Science, University of Science and Technology, Daejeon 34113, Republic of Korea, 3Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 34134, Republic of Korea |
Bibliography |
Journal of Microbiology, 56(4),209–216, 2018,
|
DOI |
10.1007/s12275-018-7457-0
|
Key Words |
MALDI-TOF, microbial identification, clinical,
environmental, whole-cell typing |
Abstract |
Advances in mass spectrometry have enabled the investigation
of various biological systems by directly analyzing diverse
sets of biomolecules (i.e., proteins, lipids, and carbohydrates),
thus making a significant impact on the life sciences field.
Over the past decade, matrix-assisted laser desorption ionization
time-of-flight mass spectrometry (MALDI-TOF MS) has
been widely utilized as a rapid and reliable method for the
identification of microorganisms. MALDI-TOF MS has come
into widespread use despite its relatively low resolving power
(full width at half maximum, FWHM: < 5,000) and its incompatibility
with tandem MS analysis, features with which other
high-resolution mass spectrometers are equipped. Microbial
identification is achieved by searching databases containing
mass spectra of peptides and proteins extracted from microorganisms
of interest, using scoring algorithms to match analyzed
spectra with reference spectra. In this paper, we give
a brief overview of the diverse applications of rapid and robust
MALDI-TOF MS-based techniques for microbial identification
in a variety of fields, such as clinical diagnosis and environmental
and food monitoring. We also describe the fundamental
principles of MALDI-TOF MS. The general specifications
of the two major MS-based microbial identification
systems available in the global market (BioTyper® and VITEK®
MS Plus) and the distribution of these instruments in Republic
of Korea are also discussed. The current review provides an
understanding of this emerging microbial identification and
classification technology and will help bacteriologists and
cell biologists take advantage of this powerful technique. |