Title |
Bacillus spongiae sp. nov., isolated from sponge of Jeju Island |
Author |
Ga-Eun Lee1, Wan-Taek Im2*, and Jin-Sook Park1* |
Address |
1Department of Biological Sciences and Biotechnology, Hannam University, Daejeon 34430, Republic of Korea, 2Department of Biotechnology, Hankyong National University, Anseong 17579, Republic of Korea |
Bibliography |
Journal of Microbiology, 56(4),217–222, 2018,
|
DOI |
10.1007/s12275-018-7511-y
|
Key Words |
Bacillus spongiae, 16S rRNA gene, polyphasic taxonomy,
sponge |
Abstract |
A Gram-reaction-positive, strictly aerobic, motile, endospore-
forming, and rod-shaped bacterial strain designated
135PIL107-10T was isolated from a sponge on Jeju Island, and
its taxonomic position was investigated using a polyphasic
approach. Strain 135PIL107-10T grew at 20–37°C (optimum
temperature, 25°C) and pH 6.0–10.0 (optimum pH, 6.0) on
marine and R2A agars. Based on 16S rRNA gene phylogeny
analysis, the novel strain formed a new branch within the genus
Bacillus of the family Bacillaceae, and formed clusters
with Bacillus thaohiensis NHI-38T (96.8%), Bacillus fengqiuensis
NPK15T (96.7%), and Bacillus songklensis CAU 1033T
(96.7%). Lower sequence similarities (97.0%) were found
with the type strains of all other recognized members of the
genus Bacillus (95.6–96.8% similarity). The G + C content
of the genomic DNA was 43.6 mol%. The predominant respiratory
quinone was menaquinone-7 and the major fatty
acids were iso-C15:0 and iso-C17:1ω10c. The overall polar lipid
patterns were diphosphatidylglycerol, phosphatidylglycerol,
and phosphatidylethanolamine. The diagnostic diamino acid
in the cell-wall peptidoglycan was meso-diaminopimelic acid.
The isolate therefore represents a novel species, for which
the name Bacillus spongiae sp. nov. is proposed, with the type
strain 135PIL107-10T (= KACC 19275T = LMG 30080T). |